Document Detail

Incorporation of cis-9,trans-11 or trans-10,cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men.
MedLine Citation:
PMID:  14754909     Owner:  NLM     Status:  MEDLINE    
This study investigated the incorporation of cis-9,trans-11 conjugated linoleic acid (c9,t11 CLA) and trans-10,cis-12-CLA (t10,c12 CLA) into plasma and peripheral blood mononuclear cell (PBMC) lipids when consumed as supplements highly enriched in these isomers. Healthy men (n = 49, age 31 +/- 8 years) consumed one, two, and four capsules containing approximately 600 mg of either c9,t11 CLA or t10,c12 CLA per capsule for sequential 8 week periods followed by a 6 week washout before consuming the alternative isomer. Both isomers were incorporated in a dose-dependent manner into plasma phosphatidylcholine (PC) (c9,t11 CLA r = 0.779, t10,c12 CLA r = 0.738; P < 0.0001) and cholesteryl ester (CE) (c9,t11 CLA r = 0.706, t10,c12 CLA r = 0.788; P < 0.0001). Only t10,c12 CLA was enriched in plasma nonesterified fatty acids. Both c9,t11 CLA and t10,c12 CLA were incorporated linearly into PBMC total lipids (r = 0.285 and r = 0.273, respectively; P < 0.0005). The highest concentrations of c9,t11 CLA and t10,c12 CLA in PBMC lipids were 3- to 4-fold lower than those in plasma PC and CE. These data suggest that the level of intake is a major determinant of plasma and PBMC CLA content, although PBMCs appear to incorporate both CLA isomers less readily.
Graham C Burdge; Berit Lupoli; Jennifer J Russell; Sabine Tricon; Samantha Kew; Tapati Banerjee; Kevin J Shingfield; David E Beever; Robert F Grimble; Christine M Williams; Parveen Yaqoob; Philip C Calder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-02-01
Journal Detail:
Title:  Journal of lipid research     Volume:  45     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-15     Completed Date:  2004-12-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  736-41     Citation Subset:  IM    
Institute of Human Nutrition, University of Southampton, Southampton, SO16 7PX UK.
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MeSH Terms
Cholesterol Esters / chemistry
Cross-Over Studies
Dietary Supplements
Double-Blind Method
Fatty Acids / chemistry
Leukocytes, Mononuclear / chemistry
Linoleic Acids / administration & dosage,  blood*,  pharmacokinetics
Linoleic Acids, Conjugated / administration & dosage,  blood*,  pharmacokinetics
Lipids / biosynthesis*
Phosphatidylcholines / chemistry
Reg. No./Substance:
0/10,12-octadecadienoic acid; 0/Cholesterol Esters; 0/Fatty Acids; 0/Linoleic Acids; 0/Linoleic Acids, Conjugated; 0/Lipids; 0/Phosphatidylcholines; 1839-11-8/9,11-linoleic acid

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