| Incorporation of alpha-linolenic acid and linoleic acid into human respiratory epithelial cell lines. | |
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MedLine Citation:
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PMID: 11521969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Animal and human studies designed to examine the effects of alpha-linolenic acid (ALA) and linoleic acid (LA) supplementation on the fatty acid composition of plasma and tissues have demonstrated a marked difference in incorporation into phospholipids of these 18-carbon precursors of the long-chain polyunsaturates. Whereas tissue phospholipid levels are linearly related to dietary ALA and LA, the levels of tissue LA can be 10-fold higher than tissue ALA even when dietary levels are equivalent. There is some dispute whether this disparity is due to ALA being more rapidly metabolized to its products or substantially oxidized by the liver, or whether LA but not ALA is readily incorporated into cellular phospholipids. We examined the level of incorporation of polyunsaturated fatty acids into human respiratory epithelial cell lines (A549, 16HBE) by determining the dose-dependent incorporation of ALA and LA as free fatty acid (5-150 microg FFA/mL). Cell membrane phospholipid ALA and LA were both increased up to approximately 20-30% total fatty acids, with a concomitant decrease predominantly in monounsaturated membrane fatty acids, before significant toxicity was observed (50 microg/mL). Our data support the concept that rather than any inherent inability by human cells to incorporate ALA into membrane phospholipids, the lack of ALA content in human and animal tissues in vivo is due to the rapid metabolism or oxidation of this fatty acid in the liver. |
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Authors:
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D L Bryan; P Hart; K Forsyth; R Gibson |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 36 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-08-27 Completed Date: 2002-01-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
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Languages: eng Pagination: 713-7 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Flinders University, South Australia, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arachidonic Acid
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metabolism Bronchi / metabolism* Cell Line, Transformed Cell Survival Docosahexaenoic Acids / metabolism Dose-Response Relationship, Drug Eicosapentaenoic Acid / metabolism Epithelial Cells / metabolism Fatty Acids, Nonesterified / metabolism Fatty Acids, Unsaturated / metabolism Humans Linoleic Acid / administration & dosage, metabolism* Membrane Lipids / metabolism Pulmonary Alveoli / metabolism* Respiratory Mucosa / cytology, metabolism* alpha-Linolenic Acid / administration & dosage, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Nonesterified; 0/Fatty Acids, Unsaturated; 0/Membrane Lipids; 1553-41-9/Eicosapentaenoic Acid; 2197-37-7/Linoleic Acid; 25167-62-8/Docosahexaenoic Acids; 463-40-1/alpha-Linolenic Acid; 506-32-1/Arachidonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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