Document Detail


Incorporation of Unnatural Non-α-Amino Acids into the N-Terminus of Proteins in a Cell-Free Translation System.
MedLine Citation:
PMID:  21956566     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Unnatural amino acid mutagenesis allows us to introduce unnatural α-amino acids into internal positions of proteins in response to expanded codons such as amber and four-base codons. To improve the unnatural amino acid mutagenesis, the incorporation of unnatural α-amino acids and non-α-amino acids into the N-terminus of proteins has been achieved using expanded initiation codons. Here, we describe the method for the incorporation of fluorescent-labeled non-α-amino acids into the N-terminus of proteins in a cell-free translation system.
Authors:
Takahiro Hohsaka
Related Documents :
17145616 - The effect of zoledronic acid on serum osteoprotegerin in early stage multiple myeloma.
23071936 - Nanocarrier-based hydrogel of betamethasone dipropionate and salicylic acid for treatme...
3097616 - Identification of interference affecting the gas-liquid chromatography analysis of valp...
24061166 - Filaggrin deficiency leads to impaired lipid profile and altered acidification pathways...
9042986 - Interaction of an aluminum-magnesium containing antacid and gastric mucus: possible con...
20487846 - Effect of co(2) on labelling of nerve and liver cells by nucleic acid precursors.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  794     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-09-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  229-39     Citation Subset:  IM    
Affiliation:
School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, Japan, hohsaka@jaist.ac.jp.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Site-specific incorporation of unnatural amino acids into proteins in Mammalian cells.
Next Document:  Site-specific modification of proteins by the staudinger-phosphite reaction.