| Incorporation of Unnatural Non-α-Amino Acids into the N-Terminus of Proteins in a Cell-Free Translation System. | |
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MedLine Citation:
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PMID: 21956566 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Unnatural amino acid mutagenesis allows us to introduce unnatural α-amino acids into internal positions of proteins in response to expanded codons such as amber and four-base codons. To improve the unnatural amino acid mutagenesis, the incorporation of unnatural α-amino acids and non-α-amino acids into the N-terminus of proteins has been achieved using expanded initiation codons. Here, we describe the method for the incorporation of fluorescent-labeled non-α-amino acids into the N-terminus of proteins in a cell-free translation system. |
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Authors:
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Takahiro Hohsaka |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 794 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-09-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 229-39 Citation Subset: IM |
Affiliation:
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School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, Japan, hohsaka@jaist.ac.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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