Document Detail

Incorporation of S-[3H]methyl-D-cysteine into the peptidoglycan of ether-treated cells of Escherichia coli.
MedLine Citation:
PMID:  1505738     Owner:  NLM     Status:  MEDLINE    
Certain D-amino acids can be incorporated into the murein sacculus of Escherichia coli apparently through a direct transpeptidation reaction independent of the normal biosynthetic pathway. Investigation of this process is important because it could lead to the identification of hitherto unknown enzymes involved in murein metabolism. However, a serious drawback is the lack of an appropriate in vitro assay. We have analysed the suitability of a system based on the incorporation of a radioactive substrate (S-[3H]methyl-D-cysteine) by ether-treated cells, a method successfully applied before to the study of murein biosynthesis. The results reported here indicate that ether-treated cells are indeed proficient in the incorporation of D-amino acids, matching closely the properties of the reaction in growing cells.
M Caparrós; V Arán; M A de Pedro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  72     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-09-21     Completed Date:  1992-09-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  139-46     Citation Subset:  IM    
Centro de Biología Molecular, C.S.I.C.-U.A.M., Facultad de Ciencias, U.A.M., Madrid, Spain.
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MeSH Terms
Amino Acids / metabolism,  pharmacology
Cysteine / analogs & derivatives*,  antagonists & inhibitors,  metabolism
Escherichia coli / drug effects,  growth & development,  metabolism*
Ether, Ethyl / pharmacology*
Peptidoglycan / drug effects,  metabolism*
Reg. No./Substance:
0/Amino Acids; 0/Peptidoglycan; 52-90-4/Cysteine; 60-29-7/Ether, Ethyl; 7728-98-5/S-methylcysteine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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