| Incorporation of S-[3H]methyl-D-cysteine into the peptidoglycan of ether-treated cells of Escherichia coli. | |
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MedLine Citation:
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PMID: 1505738 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Certain D-amino acids can be incorporated into the murein sacculus of Escherichia coli apparently through a direct transpeptidation reaction independent of the normal biosynthetic pathway. Investigation of this process is important because it could lead to the identification of hitherto unknown enzymes involved in murein metabolism. However, a serious drawback is the lack of an appropriate in vitro assay. We have analysed the suitability of a system based on the incorporation of a radioactive substrate (S-[3H]methyl-D-cysteine) by ether-treated cells, a method successfully applied before to the study of murein biosynthesis. The results reported here indicate that ether-treated cells are indeed proficient in the incorporation of D-amino acids, matching closely the properties of the reaction in growing cells. |
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Authors:
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M Caparrós; V Arán; M A de Pedro |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS microbiology letters Volume: 72 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 1992 Jun |
Date Detail:
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Created Date: 1992-09-21 Completed Date: 1992-09-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 139-46 Citation Subset: IM |
Affiliation:
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Centro de Biología Molecular, C.S.I.C.-U.A.M., Facultad de Ciencias, U.A.M., Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism,
pharmacology Cysteine / analogs & derivatives*, antagonists & inhibitors, metabolism Escherichia coli / drug effects, growth & development, metabolism* Ether, Ethyl / pharmacology* Peptidoglycan / drug effects, metabolism* Stereoisomerism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Peptidoglycan; 52-90-4/Cysteine; 60-29-7/Ether, Ethyl; 7728-98-5/S-methylcysteine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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