Document Detail


Incorporation of the HIV-1 microbicide cyanovirin-N in a food product.
MedLine Citation:
PMID:  21926631     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An urgent need exists for HIV-1 microbicides. Here, we describe the in vivo testing of lactic acid bacteria bioengineered to secrete cyanovirin-N. We fed pigtail macaques a yogurt formulation that used bioengineered strains as a starter culture. Cyanovirin-N expression could be detected in the rectal vault during and immediately after feeding. Ex vivo viral challenge of rectal tissue biopsies revealed that peak viral burden was significantly lower in tissue obtained from experimental animals compared with control animals. Formulation of candidate compounds in lactic acid bacteria and their oral administration seems to be a feasible strategy for mucosal delivery of microbicides.
Authors:
Ming Li; Dorothy L Patton; Yvonne Cosgrove-Sweeney; Deena Ratner; Lisa C Rohan; Alexander M Cole; Patrick M Tarwater; Phalguni Gupta; Bharat Ramratnam
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of acquired immune deficiency syndromes (1999)     Volume:  58     ISSN:  1944-7884     ISO Abbreviation:  J. Acquir. Immune Defic. Syndr.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-10-28     Completed Date:  2011-12-22     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  100892005     Medline TA:  J Acquir Immune Defic Syndr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  379-84     Citation Subset:  IM; X    
Affiliation:
Laboratory of Retrovirology, Division of Infectious Diseases, Department of Medicine, Rhode Island and Miriam Hospitals, Warren Alpert Medical School of Brown University, Providence, RI, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-HIV Agents / administration & dosage
Bacterial Proteins / administration & dosage*
Carrier Proteins / administration & dosage*
Feces / chemistry,  virology
Female
Food Additives / administration & dosage*
HIV Infections / prevention & control
HIV-1 / drug effects
Macaca nemestrina
Rectum / chemistry,  drug effects,  virology
Virus Replication / drug effects
Yogurt*
Grant Support
ID/Acronym/Agency:
P01 AA019072/AA/NIAAA NIH HHS; P20RR025179/RR/NCRR NIH HHS; P30 AI042853/AI/NIAID NIH HHS; P30AI042853/AI/NIAID NIH HHS; RR00166/RR/NCRR NIH HHS; T32DA013911/DA/NIDA NIH HHS; U19 AI065430/AI/NIAID NIH HHS; U19AI065430/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Anti-HIV Agents; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Food Additives; 184539-38-6/cyanovirin N
Comments/Corrections

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