Document Detail

Incorporation of aggrecan in interpenetrating network hydrogels to improve cellular performance for cartilage tissue engineering.
MedLine Citation:
PMID:  23379843     Owner:  NLM     Status:  MEDLINE    
Interpenetrating network (IPN) hydrogels were recently introduced to the cartilage tissue engineering literature, with the approach of encapsulating cells in thermally gelling agarose that is then soaked in a poly(ethylene glycol) diacrylate (PEGDA) solution, which is then photopolymerized. These IPNs possess significantly enhanced mechanical performance desirable for cartilage regeneration, potentially allowing patients to return to weight-bearing activities quickly after surgical implantation. In an effort to improve cell viability and performance, inspiration was drawn from previous studies that have elicited positive chondrogenic responses to aggrecan, the proteoglycan largely responsible for the compressive stiffness of cartilage. Aggrecan was incorporated into the IPNs in conservative concentrations (40 μg/mL), and its effect was contrasted with the incorporation of chondroitin sulfate (CS), the primary glycosaminoglycan associated with aggrecan. Aggrecan was incorporated by physical entrapment within agarose and methacrylated CS was incorporated by copolymerization with PEGDA. The IPNs incorporating aggrecan or CS exhibited over 50% viability with encapsulated chondrocytes after 6 weeks. Both aggrecan and CS improved cell viability by 15.6% and 20%, respectively, relative to pure IPNs at 6 weeks culture time. In summary, we have introduced the novel approach of including a raw material from cartilage, namely aggrecan, to serve as a bioactive signal to cells encapsulated in IPN hydrogels for cartilage tissue engineering, which led to improved performance of encapsulated chondrocytes.
Ganesh C Ingavle; Anthony W Frei; Stevin H Gehrke; Michael S Detamore
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-03-26
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  19     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-04-29     Completed Date:  2013-11-15     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1349-59     Citation Subset:  IM    
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MeSH Terms
Aggrecans / pharmacology*
Cartilage / cytology*,  drug effects,  physiology*
Cell Survival / drug effects
Chondrocytes / cytology,  drug effects,  metabolism
Compressive Strength / drug effects
DNA / metabolism
Glycosaminoglycans / metabolism
Hydrogels / pharmacology*
Hydroxyproline / metabolism
Materials Testing
Microscopy, Confocal
Sus scrofa
Tissue Engineering / methods*
Grant Support
Reg. No./Substance:
0/Aggrecans; 0/Glycosaminoglycans; 0/Hydrogels; 64082-61-7/A73025; 9007-49-2/DNA; RMB44WO89X/Hydroxyproline

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