Document Detail


Incorporation of (2-3H)glycerol into rat brain 1,2-diacyl-sn-glycero-3-phosphorylcholine and 1,2-diacyl-sn-glycerol molecular species in vivo.
MedLine Citation:
PMID:  4811214     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat brain 1,2-diacyl-sn-glycerols (diglycerides) and 1,2-diacyl-sn-glycerols obtained from 1,2-diacyl-sn-glycero-3-phosphorylcholine after treatment with phospholipase C differ markedly in carbon number distribution. 70% of the 1,2-diacyl-sn-glycerols had a total of 38 fatty acid carbon atoms, and there was no detectable change in the 1,2-diacyl-sn-glycerol mass pattern between 7 and 23 days of age. In contrast, 1,2-diacyl-sn-glycero-3-phosphorylcholine contained at most 10% of this molecular species in the brains of rats of comparable age. A small increase in the C(36) species of 1,2-diacyl-sn-glycero-3-phosphorylcholine, which is associated with myelination, was noted between 10 and 17 days. The incorporation of intracranially injected [2-(3)H]glycerol into 1,2-diacyl-sn-glycero-3-phosphoryl-choline species with polyunsaturated fatty acids containing 20 or 22 carbon atoms was greater than into the species containing only saturated and/or monoenoic fatty acids between 30 min and 24 hr. The 1,2-diacyl-sn-glycerol fractions containing polyunsaturated fatty acids had the lowest specific activity at 30 min. The specific activity of the particular 1,2-diacyl-sn-glycerol fraction containing the stearate-arachidonate pair is the lowest for 4 hr after intracranial injection of the isotope. Thus, molecular species of 1,2-diacyl-sn-glycerol and 1,2-diacyl-sn-glycero-3-phosphorylcholine differed considerably in their labeling patterns, and a direct precursor-product relationship could not be demonstrated during the time period studied.
Authors:
J F O'Brien; R L Geison
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  15     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1974 Jan 
Date Detail:
Created Date:  1974-03-28     Completed Date:  1974-03-28     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  44-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / growth & development,  metabolism*
Brain Chemistry
Chromatography, Gas
Chromatography, Thin Layer
Glycerides / biosynthesis*,  isolation & purification,  metabolism
Glycerol / metabolism*
Kinetics
Phosphatidylcholines / biosynthesis*,  isolation & purification
Phospholipases
Rats
Tritium
Chemical
Reg. No./Substance:
0/Glycerides; 0/Phosphatidylcholines; 10028-17-8/Tritium; 56-81-5/Glycerol; EC 3.1.-/Phospholipases

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