| Incorporation of (2-3H)glycerol into rat brain 1,2-diacyl-sn-glycero-3-phosphorylcholine and 1,2-diacyl-sn-glycerol molecular species in vivo. | |
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MedLine Citation:
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PMID: 4811214 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat brain 1,2-diacyl-sn-glycerols (diglycerides) and 1,2-diacyl-sn-glycerols obtained from 1,2-diacyl-sn-glycero-3-phosphorylcholine after treatment with phospholipase C differ markedly in carbon number distribution. 70% of the 1,2-diacyl-sn-glycerols had a total of 38 fatty acid carbon atoms, and there was no detectable change in the 1,2-diacyl-sn-glycerol mass pattern between 7 and 23 days of age. In contrast, 1,2-diacyl-sn-glycero-3-phosphorylcholine contained at most 10% of this molecular species in the brains of rats of comparable age. A small increase in the C(36) species of 1,2-diacyl-sn-glycero-3-phosphorylcholine, which is associated with myelination, was noted between 10 and 17 days. The incorporation of intracranially injected [2-(3)H]glycerol into 1,2-diacyl-sn-glycero-3-phosphoryl-choline species with polyunsaturated fatty acids containing 20 or 22 carbon atoms was greater than into the species containing only saturated and/or monoenoic fatty acids between 30 min and 24 hr. The 1,2-diacyl-sn-glycerol fractions containing polyunsaturated fatty acids had the lowest specific activity at 30 min. The specific activity of the particular 1,2-diacyl-sn-glycerol fraction containing the stearate-arachidonate pair is the lowest for 4 hr after intracranial injection of the isotope. Thus, molecular species of 1,2-diacyl-sn-glycerol and 1,2-diacyl-sn-glycero-3-phosphorylcholine differed considerably in their labeling patterns, and a direct precursor-product relationship could not be demonstrated during the time period studied. |
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Authors:
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J F O'Brien; R L Geison |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of lipid research Volume: 15 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1974 Jan |
Date Detail:
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Created Date: 1974-03-28 Completed Date: 1974-03-28 Revised Date: 2007-03-21 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 44-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / growth & development, metabolism* Brain Chemistry Chromatography, Gas Chromatography, Thin Layer Glycerides / biosynthesis*, isolation & purification, metabolism Glycerol / metabolism* Kinetics Phosphatidylcholines / biosynthesis*, isolation & purification Phospholipases Rats Tritium |
| Chemical | |
Reg. No./Substance:
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0/Glycerides; 0/Phosphatidylcholines; 10028-17-8/Tritium; 56-81-5/Glycerol; EC 3.1.-/Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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