Document Detail

Incomplete recoveries of fumonisins present in naturally contaminated corn foods from an immunoaffinity column.
MedLine Citation:
PMID:  19485209     Owner:  NLM     Status:  MEDLINE    
Following previous observations of apparent instability of fumonisin B1 in corn starch and corn meal, immunoaffinity column (IAC) cleanup, of the type used in the analysis of commercial starch-containing corn foods for fumonisins, was investigated. Foods analyzed for naturally occurring fumonisins B1, B2, and B3 included corn flour (3 different products), corn meal, and corn flakes. In 2 series of experiments, fractions were eluted by gravity or vacuum from narrow- or wide-bore Fumonitest IACs either with 2 x 2 mL methanol, followed by 2 mL methanol-water (8 + 2, v/v), or with 2 mL methanol, then 2 mL methanol-water (8 + 2, v/v). The ratio (%) of fumonisin B1 concentration in the first methanol eluate to the total concentration measured from all eluates in most cases varied from 25-70%. Incomplete recoveries were also observed for fumonisins B2 and B3. It is concluded that there can be a major underestimation of naturally occurring fumonisins in methods using only methanol elution for IAC cleanup, as in AOAC INTERNATIONAL Official Method 2001.04 (accuracy of these methods had been determined only by spiking the food with fumonisins). Elution with 2 x 2 mL methanol-water (8 + 2, v/v) was chosen as a practical procedure; means of 81-98% of the total fumonisin B1 concentration were found in the first eluate, except for corn flakes and 2 experiments with corn meal.
Keum Soon Oh; Peter M Scott; Soo-Hyun Chung
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  92     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2009 Mar-Apr
Date Detail:
Created Date:  2009-06-02     Completed Date:  2009-07-01     Revised Date:  2010-02-22    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  496-501     Citation Subset:  IM    
Korea Food and Drug Administration, Department of Food Safety Evaluation, Seoul 122-704, Korea.
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MeSH Terms
Chromatography, Affinity / methods*
Chromatography, High Pressure Liquid
Food Contamination / analysis*
Fumonisins / isolation & purification*,  toxicity
Zea mays / chemistry*,  toxicity
Reg. No./Substance:
0/Fumonisins; 0/Solvents; 116355-83-0/fumonisin B1; 116355-84-1/fumonisin B2; 136379-59-4/fumonisin B3; 67-56-1/Methanol; 7732-18-5/Water
Erratum In:
J AOAC Int. 2009 Nov-Dec;92(6):203A

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