Document Detail

Incomplete activation of peripheral blood dendritic cells during healthy human pregnancy.
MedLine Citation:
PMID:  21352205     Owner:  NLM     Status:  MEDLINE    
Successful pregnancy relies on the adaptation of immune responses that allow the fetus to grow and develop in the uterus despite being recognized by maternal immune cells. Dendritic cells (DCs) are central to the control of immune tolerance, and their state of activation at the maternal-decidual interface is critical to the feto-maternal immunological equilibrium. So far, the involvement of circulating DCs has been investigated poorly. Therefore, in this study we investigated whether, during healthy human pregnancy, peripheral blood DCs (PBDCs) undergo changes that may be relevant to the adaptation of maternal immune responses that allow fetal tolerance. In a cross-sectional study, we analysed PBDCs by six-colour flow cytometry on whole blood samples from 47 women during healthy pregnancy progression and 24 non-pregnant controls. We demonstrated that both myeloid and plasmacytoid PBDCs undergo a state of incomplete activation, more evident in the third trimester, characterized by increased expression of co-stimulatory molecules and cytokine production but lacking human leucocyte antigen (HLA)-DR up-regulation. To investigate the contribution of soluble circulating factors to this phenomenon, we also performed culture experiments showing that sera from pregnant women added to control DCs conditioned a similar incomplete activation that was associated with reduced DC allostimulatory capacity, supporting the in vivo relevance of our findings. We also obtained evidence that the glycoprotein hormone activin-A may contribute to DC incomplete activation. We suggest that the changes of PBDCs occurring during late pregnancy may aid the comprehension of the immune mechanisms operated by the maternal immune system to maintain fetal tolerance.
S Della Bella; S Giannelli; V Cozzi; V Signorelli; M Cappelletti; I Cetin; M L Villa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-02-24
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  164     ISSN:  1365-2249     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-11     Completed Date:  2011-06-14     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  180-92     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors; Clinical and Experimental Immunology © 2011 British Society for Immunology.
Department of Biomedical Sciences and Technologies, Lab of Immunology, Hospital 'L. Sacco', University of Milan, Italy.
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MeSH Terms
Activins / pharmacology,  physiology
Antigen Presentation
Cells, Cultured / drug effects,  immunology
Cytokines / biosynthesis,  classification,  genetics
Dendritic Cells / immunology*,  metabolism
Flow Cytometry
HLA-DR Antigens / biosynthesis,  genetics
Immune Tolerance / immunology
Leukocytes, Mononuclear / drug effects,  immunology
Lymphocyte Culture Test, Mixed
Maternal-Fetal Exchange
Pregnancy Trimester, Third
Recombinant Proteins / pharmacology
Young Adult
Reg. No./Substance:
0/Cytokines; 0/HLA-DR Antigens; 0/Recombinant Proteins; 0/activin A; 104625-48-1/Activins

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