| Inclusive estimation of complex antigen presentation functions of monocyte-derived dendritic cells differentiated under normoxia and hypoxia conditions. | |
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MedLine Citation:
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PMID: 21932134 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Dendritic cells (DCs) generated from monocytes under 20% O(2) are now used as therapeutic tools for cancer patients. However, the O(2) concentration is between 3 and 0.5% in most tissues. We evaluated these complicated functions of DCs under oxygen tensions mimicking in vivo situations. Immature DCs (imDCs) were generated from monocytes using IL-4 and GM-CSF under normoxia (20% O(2); N-imDCs) or hypoxia (1% O(2); H-imDCs). Mature DCs (mDCs) were induced with LPS. DCs were further exposed to normoxia (N/N-DCs) or hypoxia (N/H-DCs and H/H-DCs) conditions. Using a 2-D culture system, H-DCs were smaller in size than N-DCs, and H/H-DCs exhibited higher allo-T cell stimulation ability than N/N-DCs and N/H-DCs. On the other hand, motility and phagocytic ability of H/H-DCs were significantly lower than those of N/H-DCs and N/N-DCs. In a 3-D culture system, however, maturation of H/H-imDCs and N/H-imDCs was suppressed compared with N/N-imDCs as a result of their decreased motility and phagocytosis. Interestingly, silencing of HIF-1α by RNA interference decreased CD83 expression without affecting any antigen presentation abilities except for the ability to stimulate the allo-T cell population. Our data could help our understanding of DCs, especially therapeutic DCs, in vivo. |
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Authors:
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Toshitatsu Ogino; Hideya Onishi; Hiroyuki Suzuki; Takashi Morisaki; Masao Tanaka; Mitsuo Katano |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-20 |
Journal Detail:
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Title: Cancer immunology, immunotherapy : CII Volume: - ISSN: 1432-0851 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8605732 Medline TA: Cancer Immunol Immunother Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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