Document Detail


Incidence of Cryptosporidium in all patients submitting stool specimens for ova and parasite examination: monoclonal antibody IFA method.
MedLine Citation:
PMID:  3064957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of a monoclonal antibody to Cryptosporidium oocysts provides a more sensitive detection method than that seen using other diagnostic techniques. Incidence reports on this organism have been based on earlier, less-sensitive methods. In order to determine the numbers of positive patients and stool specimens, every stool specimen submitted for an ova and parasite examination was tested using the Merifluor IFA system (Meridian Diagnostics, Inc). Over a 12-mo period, 2,786 specimens were tested (1,516 patients). Positive specimens (23) were from nine known immunosuppressed patients and from four symptomatic immunocompetent patients. This represents an overall positive rate of 0.86% (patients). In those patients who were not suspected of having cryptosporidiosis (immunocompetent), diagnosis of this infection would not have been accomplished without the use of a sensitive screening method. This data, obtained over a 12-mo period, provides information for those laboratories considering the clinical relevance of screening all stool specimens for this infection. With the development of therapy, early detection of Cryptosporidium will become more important.
Authors:
L S Garcia; T C Brewer; D A Bruckner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  11     ISSN:  0732-8893     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1989-03-16     Completed Date:  1989-03-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  25-7     Citation Subset:  IM    
Affiliation:
UCLA Medical Center 90024-1713.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use
Coccidia / isolation & purification*
Cryptosporidiosis / epidemiology*
Cryptosporidium / isolation & purification*
Feces / parasitology
Fluorescent Antibody Technique
Humans
Immunologic Deficiency Syndromes / complications
Parasite Egg Count
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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