Document Detail


Development of an artificial cell, from self-organization to computation and self-reproduction.
MedLine Citation:
PMID:  21317359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This article describes the state and the development of an artificial cell project. We discuss the experimental constraints to synthesize the most elementary cell-sized compartment that can self-reproduce using synthetic genetic information. The original idea was to program a phospholipid vesicle with DNA. Based on this idea, it was shown that in vitro gene expression could be carried out inside cell-sized synthetic vesicles. It was also shown that a couple of genes could be expressed for a few days inside the vesicles once the exchanges of nutrients with the outside environment were adequately introduced. The development of a cell-free transcription/translation toolbox allows the expression of a large number of genes with multiple transcription factors. As a result, the development of a synthetic DNA program is becoming one of the main hurdles. We discuss the various possibilities to enrich and to replicate this program. Defining a program for self-reproduction remains a difficult question as nongenetic processes, such as molecular self-organization, play an essential and complementary role. The synthesis of a stable compartment with an active interface, one of the critical bottlenecks in the synthesis of artificial cell, depends on the properties of phospholipid membranes. The problem of a self-replicating artificial cell is a long-lasting goal that might imply evolution experiments.
Authors:
Vincent Noireaux; Yusuke T Maeda; Albert Libchaber
Related Documents :
20405239 - Protective effect of pacap against doxorubicin-induced cell death in cardiomyocyte cult...
19298599 - Potent antitumor activity of double-regulated oncolytic adenovirus-mediated st13 for co...
20614159 - Simvastatin overcomes the resistance to serum withdrawal-induced apoptosis of lymphocyt...
9813609 - Recognition and phagocytosis of apoptotic cells.
20981869 - Caudatin-2,6-dideoxy-3-o-methy-β-d-cymaropyranoside 1 induced apoptosis through caspas...
10761539 - Resveratrol, a natural stilbene in grapes and wine, enhances intraphagocytosis in human...
24656129 - Largen: a molecular regulator of mammalian cell size control.
12175289 - Contrast agents for confocal microscopy: how simple chemicals affect confocal images of...
20655829 - Pericellular conditions regulate extent of cell-mediated compaction of collagen gels.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-02-11
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-03     Completed Date:  2011-05-09     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3473-80     Citation Subset:  IM    
Affiliation:
University of Minnesota, 116 Church Street SE, Minneapolis, MN 55455, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Artificial Cells / cytology*,  metabolism
Cell Division*
Computational Biology*
Cytoplasmic Vesicles / metabolism
Phospholipids / metabolism
Chemical
Reg. No./Substance:
0/Phospholipids
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Motion processing with two eyes in three dimensions.
Next Document:  Identifying organic aerosol sources by comparing functional group composition in chamber and atmosph...