Document Detail


Inactivation of stable viruses in cell culture facilities by peracetic acid fogging.
MedLine Citation:
PMID:  22424718     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Looking for a robust and simple method to replace formaldehyde fumigation for the disinfection of virus-handling laboratories and facilities, we tested peracetic acid fogging as a method to inactivate stable viruses under practical conditions. Peracetic acid/hydrogen peroxide (5.8%/27.5%, 2.0 mL/m(3)) was diluted in sufficient water to achieve ≥ 70% relative humidity and was vaporized as <10 μm droplets in a fully equipped 95 m(3) laboratory unit. High titers of reovirus 3, MVM parvovirus and an avian polyomavirus were coated on frosted glass carriers and were exposed to the peracetic acid fog in various positions in the laboratory. After vaporization, a 60 min exposure time, and venting of the laboratory, no residual virus was detected on any of the carriers (detection limit <1 infectious unit/sample volume tested). The log reduction values were 9.0 for reovirus, 6.4 for MVM parvovirus, and 7.65 for the polyomavirus. After more than 10 disinfection runs within 12 months, no damage or functional impairment of electrical and electronic equipment was noted.
Authors:
Jens-Peter Gregersen; Bernhard Roth
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-15
Journal Detail:
Title:  Biologicals : journal of the International Association of Biological Standardization     Volume:  -     ISSN:  1095-8320     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9004494     Medline TA:  Biologicals     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.
Affiliation:
Novartis Vaccines and Diagnostics GmbH, Emil-von-Behring-Str. 76, D-35041 Marburg, Germany.
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