| Inactivation and injury of Yersinia enterocolitica by radiation and freezing. | |
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MedLine Citation:
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PMID: 7294784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell inactivation and cell injury by irradiation and freezing of the potentially enteropathogenic, food-borne gram-negative rod Yersinia enterocolitica strain WA was investigated. The radiation dose necessary to kill 90% of the initial population, i.e., one D-value, was 10.0, 14,3, and 24.0 krad when irradiation was carried out at 2 to 0, -18, and -75 degrees C, respectively. On the other hand, cell injury, i.e., inability to form colonies in agar containing 2.5% NaCl, was 32, 42 and 54% when cells were irradiated to one D-value at 2 to 0, -18, and -75 degrees C, respectively. Freezing alone (without irradiation) at -18 and -75 degrees C for 1 h resulted in 7 and 42% cell inactivation and 55 and 83% cell injury, respectively. These data show that given the same extent of cell inactivation, freezing caused substantially greater cell injury than radiation. For purposes of radiation sterilization, doses of 100 and 150 krad would be sufficient to inactivate 10 log cycles of Y. enterocolitica strain WA if irradiated at 2 to 0 and -18 degrees C, respectively. Presence of 2.5% NaCl may result in a further 50% reduction of the dose required to achieve sterility. |
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Authors:
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Y A EL-Zawahry; N Grecz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 42 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1981 Sep |
Date Detail:
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Created Date: 1981-12-15 Completed Date: 1981-12-15 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 464-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Dose-Response Relationship, Radiation Freezing Gamma Rays Sodium Chloride / pharmacology Yersinia / physiology*, radiation effects |
| Chemical | |
Reg. No./Substance:
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7647-14-5/Sodium Chloride |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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