Document Detail

Inactivation of hepatitis A virus and a calicivirus by high hydrostatic pressure.
MedLine Citation:
PMID:  12380746     Owner:  NLM     Status:  MEDLINE    
Potential application of high hydrostatic pressure processing (HPP) as a method for virus inactivation was evaluated. A 7-log10 PFU/ml hepatitis A virus (HAV) stock, in tissue culture medium, was reduced to nondetectable levels after exposure to more than 450 MPa of pressure for 5 min. Titers of HAV were reduced in a time- and pressure-dependent manner between 300 and 450 MPa. In contrast, poliovirus titer was unaffected by a 5-min treatment at 600 MPa. Dilution of HAV in seawater increased the pressure resistance of HAV, suggesting a protective effect of salts on virus inactivation. RNase protection experiments indicated that viral capsids may remain intact during pressure treatment, suggesting that inactivation was due to subtle alterations of viral capsid proteins. A 7-log10 tissue culture infectious dose for 50% of the cultures per ml of feline calicivirus, a Norwalk virus surrogate, was completely inactivated after 5-min treatments with 275 MPa or more. These data show that HAV and a Norwalk virus surrogate can be inactivated by HPP and suggest that HPP may be capable of rendering potentially contaminated raw shellfish free of infectious viruses.
David H Kingsley; Dallas G Hoover; Efi Papafragkou; Gary P Richards
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food protection     Volume:  65     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-16     Completed Date:  2002-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1605-9     Citation Subset:  IM    
U.S. Department of Agriculture, Microbial Food Safety Research Unit, W. W. Baker Center, Delaware State University, Dover 19901, USA.
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MeSH Terms
Caliciviridae / physiology*
Food Microbiology
Hepatitis A virus / physiology*
Hydrostatic Pressure*
Seafood / virology
Time Factors
Virus Inactivation*
Reg. No./Substance:

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