Document Detail

Inactivation of aflatoxin B1 mutagenicity by thiols.
MedLine Citation:
PMID:  6819217     Owner:  NLM     Status:  MEDLINE    
The mutagenicity of aflatoxin B1 to Salmonella typhimurium strain TA98 decreased rapidly upon exposure of aflatoxin B1 to various thiols in aqueous solution. Mutagenic activity was reduced to control values within 24 hr with N-acetyl-L-cysteine (NAC), N-2-mercaptopropionylglycine (MPG), mercaptoethanol, reduced glutathione or mercaptopropionic acid at pH values near 4. Mercaptoacetic acid, mercaptosuccinic acid, cysteine, acetyl-D,L-homocysteine thiolactone, cysteine methyl ester, D-penicillamine and beta-mercaptoethylamine were less effective. Relatively high thiol concentrations (greater than or equal to 0 . 25 M) were required to achieve complete inactivation within 24 hr with the thiols tested. The inactivation rate was strongly dependent on thiol concentration and pH, but was relatively independent of the aflatoxin concentration under the conditions examined. With MPG and NAC reaction rates were much slower at neutral pH values than at pH's between 3 and 4. HPLC and thin-layer chromatographic examination of aflatoxin B1 solutions partially inactivated with NAC revealed the formation of a new product at a rate that correlated with the disappearance of aflatoxin B1 and the loss of mutagenic activity. This reaction product has not yet been identified, but the evidence suggests that it is the product of an addition of the thiol at the difuran region of the aflatoxin.
M Friedman; C M Wehr; J E Schade; J T MacGregor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  20     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-04-15     Completed Date:  1983-04-15     Revised Date:  2006-03-13    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  887-92     Citation Subset:  IM    
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MeSH Terms
Aflatoxin B1
Aflatoxins / antagonists & inhibitors*
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Mutagenicity Tests
Mutagens / antagonists & inhibitors*
Salmonella typhimurium / genetics
Sulfhydryl Compounds / pharmacology*
Reg. No./Substance:
0/Aflatoxins; 0/Mutagens; 0/Sulfhydryl Compounds; 1162-65-8/Aflatoxin B1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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