Document Detail

Inactivation of Stat5 in mouse mammary epithelium during pregnancy reveals distinct functions in cell proliferation, survival, and differentiation.
MedLine Citation:
PMID:  15340066     Owner:  NLM     Status:  MEDLINE    
This study explored the functions of the signal transducers and activators of transcription 5a and 5b (referred to as Stat5 here) during different stages of mouse mammary gland development by using conditional gene inactivation. Mammary gland morphogenesis includes cell specification, proliferation and differentiation during pregnancy, cell survival and maintenance of differentiation throughout lactation, and cell death during involution. Stat5 is activated by prolactin, and its presence is mandatory for the proliferation and differentiation of mammary epithelium during pregnancy. To address the question of whether Stat5 is also necessary for the maintenance and survival of the differentiated epithelium, the two genes were deleted at different time points. The 110-kb Stat5 locus in the mouse was bracketed with loxP sites, and its deletion was accomplished by using two Cre-expressing transgenic lines. Loss of Stat5 prior to pregnancy prevented epithelial proliferation and differentiation. Deletion of Stat5 during pregnancy, after mammary epithelium had entered Stat5-mediated differentiation, resulted in premature cell death, indicating that at this stage epithelial cell proliferation, differentiation, and survival require Stat5.
Yongzhi Cui; Greg Riedlinger; Keiko Miyoshi; Wei Tang; Cuiling Li; Chu-Xia Deng; Gertraud W Robinson; Lothar Hennighausen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  24     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-09-01     Completed Date:  2004-10-07     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8037-47     Citation Subset:  IM    
Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0822, USA.
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MeSH Terms
Base Sequence
Cell Differentiation
Cell Division
Cell Survival
DNA / genetics
DNA-Binding Proteins / deficiency,  genetics,  metabolism*
Epithelial Cells / cytology,  metabolism
Gene Targeting
Lactation / genetics,  metabolism
Mammary Glands, Animal / cytology*,  metabolism*
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Milk Proteins*
STAT5 Transcription Factor
Trans-Activators / deficiency,  genetics,  metabolism*
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Milk Proteins; 0/STAT5 Transcription Factor; 0/Stat5a protein, mouse; 0/Trans-Activators; 9007-49-2/DNA

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