Document Detail


Inactivation of Proteolytic Enzymes from Porphyromonas gingivalis Using Light-activated Agents.
MedLine Citation:
PMID:  24590195     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Previous studies have shown one of the causative agents of periodontitis, Porphyromonas gingivalis, can be killed by red light in the presence of the light-activated antimicrobial agent toluidine blue O (TBO). The purpose of this study was to determine the effects of irradiating the organism with red light in the presence of TBO on its proteolytic enzyme activity.Suspensions of P. gingivalis were exposed to light with a wavelength of 633 nm in the presence of various concentrations of TBO. Samples were taken at various times and their proteolytic activity determined by assay of azocasein hydrolysis. On exposure to 126 J of red light in the presence of 12.5 µg/ml of TBO the proteolytic enzyme activity was reduced by 100%.The results of this study have shown that the main virulence factor of P. gingivalis, its proteolytic activity, can be inactivated by red light in the presence of TBO. This, together with the known bactericidal activity of the TBO/light combination, suggests that photodynamic therapy may prove important in reducing the effectiveness of P. gingivalis as a periodontopathogen in vivo.
Authors:
S Packer; M Bhatti; T Burns; M Wilson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lasers in medical science     Volume:  15     ISSN:  0268-8921     ISO Abbreviation:  Lasers Med Sci     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2014-03-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8611515     Medline TA:  Lasers Med Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  24-30     Citation Subset:  -    
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