Document Detail


Inactivation of human and murine norovirus by high-pressure processing.
MedLine Citation:
PMID:  21034235     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Abstract The effect of high hydrostatic pressure (HPP) was evaluated for inactivation of murine norovirus (MNV), a propagable norovirus (NoV), and human NoV genogroup II.4. Inactivation of MNV was assessed by viral culturing (50% tissue culture infectious dose [TCID(50)]) and real-time reverse-transcription-polymerase chain reaction (RT-qPCR), whereas NoV survival was determined only by RT-qPCR. A treatment of 450 MPa for 15 min at 45°C was sufficient to inactivate 6.5 log(10) of infectious MNV in culture medium as determined by TCID(50). Further, the inactivation of MNV was enhanced when pressure was applied at an initial temperature of 25°C. On the other hand, a baroprotective effect was observed when MNV suspensions were supplemented with 10 mM of CaCl(2). A 400 MPa treatment at 45°C inactivated >5 log(10) of infectious MNV, whereas the addition of CaCl(2) increased the pressure resistance of MNV, with <0.5 log(10) reduction observed. MNV decay as determined by TCID(50) was generally greater than that determined by RT-qPCR; for instance, MNV genomes were detected even after 15 min treatment at 450 MPa, with <0.5 log(10) reduction. Experiments with NoV suspensions showed that all tested HPP treatments reduced the numbers of NoV by <0.5 log(10) units as determined by RT-qPCR. Additionally, RNA of human NoV was more resistant to certain HPP treatments than the RNA of MNV.
Authors:
Gloria Sánchez; Rosa Aznar; Antonio Martínez; Dolores Rodrigo
Related Documents :
3408735 - Pressure effects on protein secondary structure and hydrogen deuterium exchange in chym...
10991465 - Pressure-induced hydrogen bonding: structure of d2s phase i'
18472025 - Effects of pressure on enzyme function of escherichia coli dihydrofolate reductase.
18447555 - A novel gas-loading system for mechanically closing of various types of diamond anvil c...
8653835 - Plasma angiotensin ii, predisposition to hypertension, and left ventricular size in hea...
21414955 - Effect of positional changes on inferior vena cava size.
Publication Detail:
Type:  Journal Article     Date:  2010-10-29
Journal Detail:
Title:  Foodborne pathogens and disease     Volume:  8     ISSN:  1556-7125     ISO Abbreviation:  Foodborne Pathog. Dis.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120121     Medline TA:  Foodborne Pathog Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-53     Citation Subset:  IM    
Affiliation:
1 Department of Biotechnology, Institute of Agrochemistry and Food Technology (IATA) , Spanish Council for Scientific Research (CSIC), Paterna, Spain .
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A Procedure for Monitoring the Presence of the Virulence Plasmid (pYV) in Yersinia pestis Under Cult...
Next Document:  Antibiotic resistance and molecular characterization of probiotic and clinical lactobacillus strains...