Document Detail


Inactivation of Escherichia coli O157:H7 in apple juice by irradiation.
MedLine Citation:
PMID:  9797319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three strains (932, Ent-C9490, and SEA13B88) of Escherichia coli O157:H7 were used to determine the effectiveness of low-dose gamma irradiation for eliminating E. coli O157:H7 from apple juice or cider and to characterize the effect of inducing pH-dependent, stationary-phase acid resistance on radiation resistance. The strains were grown in tryptic soy broth with or without 1% dextrose for 18 h to produce cells that were or were not induced to pH-dependent stationary-phase acid resistance. The bacteria were then transferred to clarified apple juice and irradiated at 2 degrees C with a cesium-137 irradiator. Non-acid-adapted cells had radiation D values (radiation doses needed to decrease a microbial population by 90%) ranging from 0.12 to 0.21 kGy. D values increased to 0.22 to 0.31 kGy for acid-adapted cells. When acid-adapted SEA13B88 cells were tested in five apple juice brands having different levels of suspended solids (absorbances ranging from 0.04 to 2.01 at 550 nm), radiation resistance increased with increasing levels of suspended solids, with D values ranging from 0.26 to 0.35 kGy. Based on these results, a dose of 1.8 kGy should be sufficient to achieve the 5D inactivation of E. coli recommended by the National Advisory Committee for Microbiological Criteria for Foods.
Authors:
R L Buchanan; S G Edelson; K Snipes; G Boyd
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  64     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-01-14     Completed Date:  1999-01-14     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4533-5     Citation Subset:  IM    
Affiliation:
Food Safety Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA. rbuchana@bangate.fda.gov
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MeSH Terms
Descriptor/Qualifier:
Beverages / microbiology*
Dose-Response Relationship, Radiation
Escherichia coli O157 / growth & development,  radiation effects*
Food Irradiation*
Fruit / microbiology*
Gamma Rays
Hydrogen-Ion Concentration
Radiation Tolerance
Species Specificity
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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