Document Detail


Inactivation of Enterobacter sakazakii in reconstituted infant formula by trans-cinnamaldehyde.
MedLine Citation:
PMID:  19091435     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enterobacter sakazakii is an emerging pathogen which causes a life-threatening form of meningitis, necrotizing colitis and meningoencephalitis in neonates and children. Epidemiological studies implicate dried infant formula as the principal source of the pathogen. Trans-cinnamaldehyde is a major component of bark extract of cinnamon. It is classified as generally recognized as safe (GRAS) by the U.S. Food and Drug Administration, and is approved for use in food (21 CFR 182.60). The objective of this study was to determine the antibacterial effect of trans-cinnamaldehyde on E. sakazakii in reconstituted infant formula. A 5-strain mixture of E. sakazakii was inoculated into 10 ml samples of reconstituted infant formula (at 6.0 log CFU/ml) containing 0%, 0.15%, 0.3% or 0.5% trans-cinnamaldehyde. The samples were incubated at 37, 23, 8 or 4 degrees C for 0, 6, 10 and 24 h, and the surviving populations of E. sakazakii at each sampling time were enumerated. In addition, potential cytotoxicity of trans-cinnamaldehyde, if any, was determined on human embryonic intestinal cells (INT-407). The treatments containing trans-cinnamaldehyde significantly reduced (P<0.05) the population of E. sakazakii, compared to the controls. Trans-cinnamaldehyde (0.5%) reduced the pathogen to undetectable levels by 4 h of incubation at 37 or 23 degrees C and 10 h of incubation at 8 or 4 degrees C, respectively. Trans-cinnamaldehyde produced no cytotoxic effects on human embryonic intestinal cells at the tested concentrations. Results indicate that trans-cinnamaldehyde could potentially be used to kill E. sakazakii in reconstituted infant formula, however sensory studies are warranted before recommending its use.
Authors:
Mary Anne Roshni Amalaradjou; Thomas A Hoagland; Kumar Venkitanarayanan
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Publication Detail:
Type:  Journal Article     Date:  2008-11-21
Journal Detail:
Title:  International journal of food microbiology     Volume:  129     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-05     Completed Date:  2009-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  146-9     Citation Subset:  IM    
Affiliation:
Department of Animal Science, Unit-4040, University of Connecticut, Storrs, Connecticut 06269, USA.
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MeSH Terms
Descriptor/Qualifier:
Acrolein / adverse effects,  analogs & derivatives*,  pharmacology
Anti-Bacterial Agents / adverse effects,  pharmacology*
Cells, Cultured
Cinnamomum zeylanicum / chemistry
Colony Count, Microbial
Consumer Product Safety
Enterobacter sakazakii / drug effects*,  growth & development
Food Contamination / analysis*,  prevention & control
Humans
Infant
Infant Food / microbiology*
Infant Formula
Infant, Newborn
Taste
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 104-55-2/cinnamic aldehyde; 107-02-8/Acrolein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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