| Inactivated Sendai-virus-mediated fusion improves early development of cloned bovine embryos by avoiding endoplasmic-reticulum-stress-associated apoptosis. | |
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MedLine Citation:
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PMID: 21791184 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Somatic cell nuclear transfer (SCNT) is a powerful tool, not only for producing cloned animals, but also in revealing various early developmental events. However, relatively little is known regarding the biological events and underlying mechanism(s) directly associated with early development of SCNT embryos. Here, we show that production of high-quality bovine SCNT blastocysts is dependent on the method used for fusion and the associated reduction in endoplasmic reticulum (ER) stress. During fusion between the donor cell and the enucleated oocyte, electrofusion triggers spontaneous oocyte activation, accompanied by an increase in intracellular Ca(2+) and improper nuclear remodelling. These events can be greatly reduced by the use of Sendai virus (SV)-mediated fusion. Moreover, SV-SCNT improves the blastulation rate and blastocyst quality, defined by the number and ratio of inner cell mass and trophectoderm cells in each blastocyst, in comparison with electrofusion-mediated SCNT (E-SCNT). Interestingly, expression of ER-stress-associated genes and blastomere apoptosis were significantly increased in E-SCNT embryos. These increases could be reversed by inhibition of ER stress or by using the SV-mediated fusion method. Collectively, these results indicate that SV-mediated fusion improves the developmental competence and quality of SCNT blastocysts, by reducing ER-stress-associated apoptosis. |
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Authors:
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Bong-Seok Song; Ji-Su Kim; Seung-Bin Yoon; Kyu-Sun Lee; Deog-Bon Koo; Dong-Seok Lee; Young-Kug Choo; Jae-Won Huh; Sang-Rae Lee; Sun-Uk Kim; Sang-Hyun Kim; Hwan Mook Kim; Kyu-Tae Chang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Reproduction, fertility, and development Volume: 23 ISSN: 1031-3613 ISO Abbreviation: Reprod. Fertil. Dev. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8907465 Medline TA: Reprod Fertil Dev Country: Australia |
Other Details:
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Languages: eng Pagination: 826-36 Citation Subset: IM |
Affiliation:
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National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, Republic of Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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