| Inability of the standardized disk agar-diffusion test to measure susceptibility of the fluorescent group of pseudomonads to gentamicin. | |
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MedLine Citation:
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PMID: 101077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Four hundred thirteen clinical isolates of fluorescent pseudomonads consisting of 286 Pseudomonas aeruginosa and 127 organisms of the Pseudomonas fluorescent group were tested for susceptibilities to gentamicin by the broth-dilution method and by the standardized disk agar-diffusion test, using Mueller-Hinton agar from three sources. Different regression lines and significantly different mean inhibition zone diameters were found for the three Mueller-Hinton agars. Single zone-diameter breakpoints of either 13 or 16 mm for the standardized disk agar-diffusion test produced unacceptable rates of false-sensitive and false-resistant interpretations. When error rates were limited by using zone-diameter breakpoints determined by the method of error rate-bound analysis, the percentage of indeterminate interpretations became so large as to make the standardized disk agar-diffusion test impractical. It is concluded that the standardized disk agar-diffusion test should not be used for testing susceptibility of the fluorescent pseudomonads to gentamicin, and presumably other aminoglycosides. |
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Authors:
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B F Woolfrey; W A Ramadei; C O Quall |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of clinical pathology Volume: 70 ISSN: 0002-9173 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 1978 Sep |
Date Detail:
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Created Date: 1978-12-29 Completed Date: 1978-12-29 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 337-42 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agar Gentamicins / pharmacology* Microbial Sensitivity Tests / standards* Pseudomonas / drug effects* Pseudomonas aeruginosa / drug effects Pseudomonas fluorescens / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Gentamicins; 9002-18-0/Agar |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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