| In vivo two-photon imaging reveals monocyte-dependent neutrophil extravasation during pulmonary inflammation. | |
| | |
MedLine Citation:
|
PMID: 20923880 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Immune-mediated pulmonary diseases are a significant public health concern. Analysis of leukocyte behavior in the lung is essential for understanding cellular mechanisms that contribute to normal and diseased states. Here, we used two-photon imaging to study neutrophil extravasation from pulmonary vessels and subsequent interstitial migration. We found that the lungs contained a significant pool of tissue-resident neutrophils in the steady state. In response to inflammation produced by bacterial challenge or transplant-mediated, ischemia-reperfusion injury, neutrophils were rapidly recruited from the circulation and patrolled the interstitium and airspaces of the lung. Motile neutrophils often aggregated in dynamic clusters that formed and dispersed over tens of minutes. These clusters were associated with CD115(+) F4/80(+) Ly6C(+) cells that had recently entered the lung. The depletion of blood monocytes with clodronate liposomes reduced neutrophil clustering in the lung, but acted by inhibiting neutrophil transendothelial migration upstream of interstitial migration. Our results suggest that a subset of monocytes serve as key regulators of neutrophil extravasation in the lung and may be an attractive target for the treatment of inflammatory pulmonary diseases. |
| | |
Authors:
|
Daniel Kreisel; Ruben G Nava; Wenjun Li; Bernd H Zinselmeyer; Baomei Wang; Jiaming Lai; Robert Pless; Andrew E Gelman; Alexander S Krupnick; Mark J Miller |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-05 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-10-20 Completed Date: 2010-11-22 Revised Date: 2011-08-01 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 18073-8 Citation Subset: IM |
Affiliation:
|
Department of Pathology and Immunology, Washington University, St. Louis, MO 63110, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chemotaxis, Leukocyte Lung / cytology* Mice Microscopy / methods* Monocytes / cytology* Neutrophils / cytology* Photons |
| Grant Support | |
ID/Acronym/Agency:
|
1K08HL083983/HL/NHLBI NIH HHS; 1R01HL094601/HL/NHLBI NIH HHS; AI077600/AI/NIAID NIH HHS; K08 CA131097-04/CA/NCI NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Heterogeneous myocyte enhancer factor-2 (Mef2) activation in myocytes predicts focal scarring in hyp...
Next Document: Virus engineering: functionalization and stabilization.