| In vivo transcranial cavitation threshold detection during ultrasound-induced blood-brain barrier opening in mice. | |
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MedLine Citation:
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PMID: 20876972 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The in vivo cavitation response associated with blood-brain barrier (BBB) opening as induced by transcranial focused ultrasound (FUS) in conjunction with microbubbles was studied in order to better identify the underlying mechanism in its noninvasive application. A cylindrically focused hydrophone, confocal with the FUS transducer, was used as a passive cavitation detector (PCD) to identify the threshold of inertial cavitation (IC) in the presence of Definity® microbubbles (mean diameter range: 1.1-3.3 µm, Lantheus Medical Imaging, MA, USA). A vessel phantom was first used to determine the reliability of the PCD prior to in vivo use. A cerebral blood vessel was simulated by generating a cylindrical channel of 610 µm in diameter inside a polyacrylamide gel and by saturating its volume with microbubbles. The microbubbles were sonicated through an excised mouse skull. Second, the same PCD setup was employed for in vivo noninvasive (i.e. transdermal and transcranial) cavitation detection during BBB opening. After the intravenous administration of Definity® microbubbles, pulsed FUS was applied (frequency: 1.525 or 1.5 MHz, peak-rarefactional pressure: 0.15-0.60 MPa, duty cycle: 20%, PRF: 10 Hz, duration: 1 min with a 30 s interval) to the right hippocampus of twenty-six (n = 26) mice in vivo through intact scalp and skull. T1 and T2-weighted MR images were used to verify the BBB opening. A spectrogram was generated at each pressure in order to detect the IC onset and duration. The threshold of BBB opening was found to be at a 0.30 MPa peak-rarefactional pressure in vivo. Both the phantom and in vivo studies indicated that the IC pressure threshold had a peak-rarefactional amplitude of 0.45 MPa. This indicated that BBB opening may not require IC at or near the threshold. Histological analysis showed that BBB opening could be induced without any cellular damage at 0.30 and 0.45 MPa. In conclusion, the cavitation response could be detected without craniotomy in mice and IC may not be required for BBB opening at relatively low pressures. |
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Authors:
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Yao-Sheng Tung; Fotios Vlachos; James J Choi; Thomas Deffieux; Kirsten Selert; Elisa E Konofagou |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-09-29 |
Journal Detail:
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Title: Physics in medicine and biology Volume: 55 ISSN: 1361-6560 ISO Abbreviation: Phys Med Biol Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-06 Completed Date: 2011-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401220 Medline TA: Phys Med Biol Country: England |
Other Details:
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Languages: eng Pagination: 6141-55 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Columbia University, New York, NY, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood-Brain Barrier / metabolism*, ultrasonography* Gases / metabolism* Magnetic Resonance Imaging Male Mice Mice, Inbred C57BL Phantoms, Imaging Reproducibility of Results Ultrasonics* |
| Grant Support | |
ID/Acronym/Agency:
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2R21EY018505/EY/NEI NIH HHS; R01EB009041/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Gases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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