Document Detail

In vivo tracking of dual-labeled mesenchymal stem cells homing into the injured common carotid artery.
MedLine Citation:
PMID:  19685506     Owner:  NLM     Status:  MEDLINE    
The aim of this study is to conduct in vivo, noninvasive magnetic resonance imaging of labeled rat bone mesenchymal stem cells (BMSCs) as they home into the site of injured common carotid artery following allograft transplantation. Our study was approved by the Institutional Committee on Animal Research. Purified rat BMSCs were dual labeled with superparamagnetic iron oxide (SPIO) particle and fluorescent DiI dye, and subsequently transplanted into recipient rats injured in the left common carotid arteries. Immediately before and 3 hr, 3, 7 and 12 days after transplantation, the labeled cells were monitored in vivo using a 7T micromagnetic resonance imaging (7T micro-MRI) scanner. The signal-to-noise ratios (SNRs) at the injured sites were corroborated with histological examination using Prussian blue staining and fluorescent imaging. Rat BMSCs were labeled with SPIO and DiI at 100% efficiency. When compared with the baseline level before transplantation, the SNR decreased significantly on Days 3 and 7 after injection in the experimental group (Dunnet t test, P < 0.05), whereas insignificant differences were observed after 3 hr and 12 days (Dunnet t test, P > 0.05). In the control group, no significant differences in SNR were found among different time points (ANOVA, P > 0.05). Histological analyses illustrated that red fluorescence and Prussian blue-positive cells were mainly distributed around the lesion areas of injured common carotid arteries. Rat BMSCs can be efficiently labeled with SPIO and DiI, and the directional homing of labeled cells to the site of injured common carotid arteries after intravascular transplantation could be tracked in vivo with 7T micro-MRI.
Ai Hong Cao; Hong Jian Shi; Yu Zhang; Gao Jun Teng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anatomical record (Hoboken, N.J. : 2007)     Volume:  292     ISSN:  1932-8494     ISO Abbreviation:  Anat Rec (Hoboken)     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-29     Completed Date:  2009-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101292775     Medline TA:  Anat Rec (Hoboken)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1677-83     Citation Subset:  IM    
Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
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MeSH Terms
Carbocyanines / diagnostic use*
Carotid Artery Injuries / pathology,  surgery*
Carotid Artery, Common / pathology
Cell Survival
Ferrocyanides / diagnostic use
Fluorescent Dyes / diagnostic use*
Magnetic Resonance Imaging
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells / cytology*,  physiology
Metal Nanoparticles / diagnostic use*
Microscopy, Fluorescence
Rats, Sprague-Dawley
Transplantation, Homologous
Reg. No./Substance:
0/Carbocyanines; 0/Ferrocyanides; 0/Fluorescent Dyes; 14038-43-8/ferric ferrocyanide; 40957-95-7/3,3'-dioctadecylindocarbocyanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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