| In vivo restriction. Sequence and structure of endonuclease II-dependent cleavage sites in bacteriophage T4 DNA. | |
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MedLine Citation:
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PMID: 1744134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endonuclease II of bacteriophage T4 is required for in vivo restriction of cytosine-containing DNA from its host, Escherichia coli, (as well as from phage mutants lacking cytosine modification), normally the first step in the reutilization of host DNA nucleotides for synthesis of phage DNA in infected cells. The phage cytosine-DNA is fragmented incompletely to yield genetically defined fragments. This restriction is different from that of type I, II, or III restriction enzymes. We have located seven major endonuclease II-dependent restriction sites in the T4 genome, of which three were analyzed in detail; in addition, abundant sites were cleaved in less than or equal to 5% of all molecules. Sites I, II, and III shared the sequence 5'-CCGNNTTGGC-3' and were cleaved in about 25% (I and III) and 65% (II) of all molecules, predominantly staggered around the first or second of the central unspecified base pairs to yield fragments with one 5' base. The less frequently cleaved sites I and III deviated from site II in predicted helical structure when viewed from the consensus strand, and in sequence when viewed from the opposite strand. Thus, interaction with a particular helical structure as well as recognition of the bases in DNA appears important for efficient cleavage. |
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Authors:
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M Krabbe; K Carlson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 266 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1991 Dec |
Date Detail:
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Created Date: 1992-01-13 Completed Date: 1992-01-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 23407-15 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Uppsala Biochemical Center, Sweden. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M55199; M55200; M55201; M75725; M96682; S66610; S66768; S69482; S69489; S69493 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA, Viral / metabolism* Deoxyribonuclease I / metabolism* Molecular Sequence Data Nucleic Acid Conformation Restriction Mapping Substrate Specificity T-Phages / enzymology*, genetics |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; EC 3.1.21.1/Deoxyribonuclease I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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