Document Detail

In vivo response of neutrophils and epithelial cells to lipopolysaccharide injected into the monkey ileum.
MedLine Citation:
PMID:  12432449     Owner:  NLM     Status:  MEDLINE    
Since few previous studies have investigated the in vivo response of intestinal mucosa to the luminally administered lipopolysaccharide (LPS), we examined the cellular localization of exogenously applied LPS in the intestinal mucosa and the expression of Toll-like receptor (TLR) and IL-1 receptor-associated kinase (IRAK) in the epithelial cells of monkey ileum. FITC-labeled LPS was injected into the lumen of monkey ileum. Thirty minutes after the LPS injection, the ileal tissue was fixed and localization of FITC fluorescence in the ileal mucosa was examined. We applied Factor C immunohistochemistry to demonstrate the bioactivity of LPS taken up by the mucosal tissue. The expression of TLR4 and IRAK-1 in the epithelial cells was also examined by immunohistochemistry. FITC fluorescence was detected in the cells migrated into the epithelium and those in the lamina propria. The FITC-labeling cells were completely overlapped with the Factor C immunoreactive cells. These FITC-labeling/Factor C-positive cells were identified as neutrophils by the immunoelectron microscopic analysis. TLR4 and IRAK-1 were expressed at the apical membrane of the epithelial cells in the ileum of both control and FITC-LPS injected animals. These results suggest that intraluminal injection of LPS stimulates the transmigration of neutrophils into the epithelium and these neutrophils may uptake luminally applied LPS and possibly inactivate the enterotoxin. Expression of TLR4 and IRAK-1 in the epithelial cells suggests that epithelial cells may react to LPS and produce chemoattractant mediator to induce the neutrophil chemotaxis.
Hirotsugu Imaeda; Hiroshi Yamamoto; Atsushi Takaki; Mineko Fujimiya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-10-03
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  118     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-11-14     Completed Date:  2003-06-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  381-8     Citation Subset:  IM    
Department of Anatomy, Shiga University of Medical Science, Otsu, Shiga, 520-2192, Japan.
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MeSH Terms
Enzyme Precursors / metabolism,  ultrastructure
Epithelial Cells / drug effects,  metabolism*,  ultrastructure
Fluorescein-5-isothiocyanate / diagnostic use
Ileum / cytology,  metabolism*
Interleukin-1 Receptor-Associated Kinases
Intestinal Mucosa / drug effects,  metabolism*,  ultrastructure
Lipopolysaccharides / administration & dosage,  pharmacology*
Membrane Glycoproteins / metabolism,  ultrastructure
Microscopy, Immunoelectron
Neutrophils / drug effects,  metabolism*,  ultrastructure
Protein Kinases / metabolism,  ultrastructure
Receptors, Cell Surface / metabolism,  ultrastructure
Serine Endopeptidases / metabolism,  ultrastructure
Time Factors
Toll-Like Receptor 4
Toll-Like Receptors
Reg. No./Substance:
0/Enzyme Precursors; 0/Lipopolysaccharides; 0/Membrane Glycoproteins; 0/Receptors, Cell Surface; 0/Toll-Like Receptor 4; 0/Toll-Like Receptors; 3326-32-7/Fluorescein-5-isothiocyanate; EC 2.7.-/Protein Kinases; EC Receptor-Associated Kinases; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.-/factor C

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