Document Detail


In vivo resolution of multiexponential decays of multiple near-infrared molecular probes by fluorescence lifetime-gated whole-body time-resolved diffuse optical imaging.
MedLine Citation:
PMID:  17711779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The biodistribution of two near-infrared fluorescent agents was assessed in vivo by time-resolved diffuse optical imaging. Bacteriochlorophyll a (BC) and cypate-glysine-arginine-aspartic acid-serine-proline-lysine-OH (Cyp-GRD) were administered separately or combined to mice with subcutaneous xenografts of human breast adenocarcinoma and slow-release estradiol pellets for improved tumor growth. The same excitation (780 nm) and emission (830 nm) wavelengths were used to image the distinct fluorescence lifetime distribution of the fluorescent molecular probes in the mouse cancer model. Fluorescence intensity and lifetime maps were reconstructed after raster-scanning whole-body regions of interest by time-correlated single-photon counting. Each captured temporal point-spread function (TPSF) was deconvolved using both a single and a multiexponental decay model to best determine the measured fluorescence lifetimes. The relative signal from each fluorophore was estimated for any region of interest included in the scanned area. Deconvolution of the individual TPSFs from whole-body fluorescence intensity scans provided corresponding lifetime images for comparing individual component biodistribution. In vivo fluorescence lifetimes were determined to be 0.8 ns (Cyp-GRD) and 2 ns (BC). This study demonstrates that the relative biodistribution of individual fluorophores with similar spectral characteristics can be compartmentalized by using the time-domain fluorescence lifetime gating method.
Authors:
Walter Akers; Frederic Lesage; Dewey Holten; Samuel Achilefu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Molecular imaging     Volume:  6     ISSN:  1535-3508     ISO Abbreviation:  Mol Imaging     Publication Date:    2007 Jul-Aug
Date Detail:
Created Date:  2007-08-22     Completed Date:  2008-04-04     Revised Date:  2011-04-07    
Medline Journal Info:
Nlm Unique ID:  101120118     Medline TA:  Mol Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-46     Citation Subset:  IM    
Affiliation:
Optical Radiology Lab, Department of Radiology, Washington University School of Medicine, St Louis, MO 63146, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteriochlorophylls / chemistry,  metabolism,  pharmacology
Cell Line, Tumor
Fluorescence
Humans
Injections, Intravenous
Male
Mice
Mice, Nude
Molecular Probes / analysis*,  chemistry
Neoplasm Transplantation
Neoplasms / pathology
Peptides / chemistry,  metabolism,  pharmacology
Spectrophotometry, Infrared
Time Factors
Whole Body Imaging / methods*
Grant Support
ID/Acronym/Agency:
R01 CA109754/CA/NCI NIH HHS; R01 EB1430/EB/NIBIB NIH HHS; R24 CA83060/CA/NCI NIH HHS; R33 CA100972/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Bacteriochlorophylls; 0/Molecular Probes; 0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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