Document Detail

In vivo regeneration of cut nerves encased in silicone tubes: growth across a six-millimeter gap.
MedLine Citation:
PMID:  7086464     Owner:  NLM     Status:  MEDLINE    
We describe an experimental in vivo system for studying peripheral nerve regeneration, in which the proximal stump of a transected nerve regrows through a transparent silicone chamber toward the distal stump. Physical separation permits examination of the effects of the humoral and/or cellular influences from the distal stump on regenerating fibers before they invade the distal segment itself. A small segment of the rat sciatic nerve was resected, leaving a 6 mm gap which was then encased by a cylindrical silicone chamber. Within the first weeks, a nerve trunk regenerated along the central axis of the chamber bridged the gap between the proximal and distal stumps. When the distal nerve stump was omitted from the distal opening of the chamber, only a thin structure with a few small-caliber fibers extended across the gap. In each instance regenerating nerve appeared as a cord-like structure completely surrounded by clear fluid, a feature which permits easy collection of the extracellular fluid for analysis of its chemical properties and biological activity. This feature also allows in vivo manipulation of the humoral environment in which nerve regeneration occurs.
G Lundborg; R H Gelberman; F M Longo; H C Powell; S Varon
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuropathology and experimental neurology     Volume:  41     ISSN:  0022-3069     ISO Abbreviation:  J. Neuropathol. Exp. Neurol.     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-08-26     Completed Date:  1982-08-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985192R     Medline TA:  J Neuropathol Exp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  412-22     Citation Subset:  IM    
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MeSH Terms
Microsurgery / instrumentation
Nerve Growth Factors / physiology
Nerve Regeneration*
Rats, Inbred Strains
Sciatic Nerve / anatomy & histology*,  physiology,  ultrastructure
Grant Support
Reg. No./Substance:
0/Nerve Growth Factors; 0/Silicones

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