| In vivo regeneration of cut nerves encased in silicone tubes: growth across a six-millimeter gap. | |
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MedLine Citation:
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PMID: 7086464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe an experimental in vivo system for studying peripheral nerve regeneration, in which the proximal stump of a transected nerve regrows through a transparent silicone chamber toward the distal stump. Physical separation permits examination of the effects of the humoral and/or cellular influences from the distal stump on regenerating fibers before they invade the distal segment itself. A small segment of the rat sciatic nerve was resected, leaving a 6 mm gap which was then encased by a cylindrical silicone chamber. Within the first weeks, a nerve trunk regenerated along the central axis of the chamber bridged the gap between the proximal and distal stumps. When the distal nerve stump was omitted from the distal opening of the chamber, only a thin structure with a few small-caliber fibers extended across the gap. In each instance regenerating nerve appeared as a cord-like structure completely surrounded by clear fluid, a feature which permits easy collection of the extracellular fluid for analysis of its chemical properties and biological activity. This feature also allows in vivo manipulation of the humoral environment in which nerve regeneration occurs. |
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Authors:
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G Lundborg; R H Gelberman; F M Longo; H C Powell; S Varon |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neuropathology and experimental neurology Volume: 41 ISSN: 0022-3069 ISO Abbreviation: J. Neuropathol. Exp. Neurol. Publication Date: 1982 Jul |
Date Detail:
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Created Date: 1982-08-26 Completed Date: 1982-08-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985192R Medline TA: J Neuropathol Exp Neurol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 412-22 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Microsurgery / instrumentation Nerve Growth Factors / physiology Nerve Regeneration* Rats Rats, Inbred Strains Sciatic Nerve / anatomy & histology*, physiology, ultrastructure Silicones |
| Grant Support | |
ID/Acronym/Agency:
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NS-14162/NS/NINDS NIH HHS; NS-16349/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nerve Growth Factors; 0/Silicones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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