Document Detail


In-vivo quantification of primary microRNA processing by Drosha with a luciferase based system.
MedLine Citation:
PMID:  21352811     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The RNAse III Drosha is responsible for the first step of microRNA maturation, the cleavage of primary miRNA to produce the precursor miRNA. Processing by Drosha is finely regulated and influences the amount of mature microRNA in a cell. We describe in the present work a method to quantify Drosha processing activity in-vivo, which is applicable to any microRNA. With respect to other methods for measuring Drosha activity, our system is faster and scalable, can be used with any cellular system and does not require cell sorting or use of radioactive isotopes. This system is useful to study regulation of Drosha activity in physiological and pathological conditions.
Authors:
Danilo Allegra; Daniel Mertens
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-22
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Department of Internal Medicine III, University Hospital, Albert-Einstein-Allee 23, 89081 Ulm, Germany; Cooperation unit "Mechanisms of Leukemogenesis" - B061, DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
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