| In vivo predegeneration of peripheral nerves: an effective technique to obtain activated Schwann cells for nerve conduits. | |
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MedLine Citation:
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PMID: 10476679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vivo predegeneration of peripheral nerves is presented as a convenient and effective method to obtain activated Schwann cells and an enhanced cell yield following in vitro cultivation. The experiments conducted in rats were aimed at clinical use in gaining Schwann cell suspensions for filling artificial conduits in order to bridge peripheral nerve gaps. The rat sciatic nerve used as a model was transected distally to the spinal ganglia. Predegeneration in vivo was allowed to take place for 1, 2, 3 and 4 days and up to 1, 2 and 3 weeks. The nerve was then resected and prepared for cell cultivation. Schwann cells cultivated from the contralateral untreated nerve served as control. Immunostaining for S100, nerve growth factor receptor and the adhesion molecules N-cadherin and L1 was used to characterize the general state of the cultures. Viability was assessed by fluorescein fluorescence staining, and the proliferation index was determined by bromodeoxyuridine-DNA incorporation. The Schwann cells from predegenerated nerves revealed an increased proliferation rate compared to the control, whereas fibroblast contamination was decreased. Best results were obtained 1 week after predegeneration. |
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Authors:
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G Keilhoff; H Fansa; W Schneider; G Wolf |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 89 ISSN: 0165-0270 ISO Abbreviation: J. Neurosci. Methods Publication Date: 1999 Jul |
Date Detail:
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Created Date: 1999-10-18 Completed Date: 1999-10-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 17-24 Citation Subset: IM |
Affiliation:
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Institute of Medical Neurobiology, University of Magdeburg, Germany. gerburg.keilhoff@medizin.uni-magdeburg.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bromodeoxyuridine Cell Culture Techniques / methods Cell Division Cell Separation / methods Cell Survival Cells, Cultured Ganglia, Spinal / physiology Immunohistochemistry / methods Male Nerve Degeneration Rats Rats, Wistar S100 Proteins / analysis Schwann Cells / cytology*, physiology* Sciatic Nerve / cytology*, physiology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/S100 Proteins; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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