| In-vivo nitrosation of amines in mice by inhaled nitrogen dioxide and inhibition of biosynthesis of N-nitrosamines. | |
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MedLine Citation:
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PMID: 6533019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inhalation of nitrogen dioxide (NO2) by mice administered orally morpholine (MOR) or dimethylamine (DMA) resulted in the biosynthesis of N-nitrosomorpholine (NMOR) or N-nitrosodimethylamine (NDMA), respectively, as determined by the analysis of frozen whole-mouse powder, using gas chromatography with a Thermal Energy Analyzer detector. Significant levels of NMOR were detected following exposure of mice to 0.38 mg/m3 NO2 for 0.5 h (26 ng NMOR/mouse) and there was a two-fold increase when NO2 exposure was extended to 4 h. NMOR levels also increased in a time-dependent manner at 28.4 and 47.3 mg/m3 NO2 exposure levels, reaching a maximum of 450 and 725 ng NMOR/mouse, respectively, at 4 h. Oral administration of sodium ascorbate (50-250 mg), ammonium sulfamate (50-100 mg) or DL-alpha-tocopherol (67-167 mg) immediately after MOR or DMA, but prior to NO2 exposure, significantly inhibited both NMOR and NDMA biosynthesis, sulfamate being the most effective (greater than 90% NMOR and NDMA inhibition), followed by ascorbate (83-90% NMOR and 58-90% NDMA inhibition) and alpha-tocopherol (22-42% NMOR and 46-69% NDMA inhibition). Low levels of NDMA were found in untreated control mice (less than 13 ng/mouse) and in most samples of commercially obtained animal feed (10-15 micrograms/kg); NMOR, however, was not detectable or was detected in negligible amounts in these cases. Various control experiments indicated that most of the recovered nitrosamine resulted from in-vivo nitrosation in mice, with only up to 1-2% of NMOR and approximately 10% of NDMA yields being attributed to artefact formation, possibly during work-up of the mouse-powder samples. |
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Authors:
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Z M Iqbal |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: IARC scientific publications Volume: - ISSN: 0300-5038 ISO Abbreviation: IARC Sci. Publ. Publication Date: 1984 |
Date Detail:
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Created Date: 1985-06-20 Completed Date: 1985-06-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8009542 Medline TA: IARC Sci Publ Country: FRANCE |
Other Details:
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Languages: eng Pagination: 291-300 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Gas Dimethylamines / metabolism, pharmacology Dimethylnitrosamine / metabolism Mice Morpholines / metabolism, pharmacology Nitrogen Dioxide / metabolism* Nitrosamines / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Dimethylamines; 0/Morpholines; 0/Nitrosamines; 10102-44-0/Nitrogen Dioxide; 59-89-2/N-nitrosomorpholine; 62-75-9/Dimethylnitrosamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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