Document Detail

In vivo near-infrared monitoring of nitric oxide production and tissue oxygen sufficiency in rat liver allografts during the acute rejection reaction.
MedLine Citation:
PMID:  11271303     Owner:  NLM     Status:  MEDLINE    
We established a new technique of in vivo near-infrared (NIR) spectroscopy that can estimate both nitric oxide (NO) production and tissue oxygen sufficiency in living organs during the alloimmune response. The present study was aimed at evaluating the potential of this technique for monitoring the rejection response utilizing the rat model of orthotopic liver transplantation without arterialization. The relative changes of nitrosyl-hemoglobin, oxyhemoglobin and oxidized-cytochrome oxidase in the graft livers were quantified by use of this method. Nitrosyl-hemoglobin in the allogenic grafts was elevated at the onset of the rejection response and was suppressed when the rejection reaction was treated by the administration of 15-deoxyspergualin. Oxy-hemoglobin and oxidized-cytochrome oxidase were decreased in accordance with parenchymal disorder determined histologically. These results demonstrated that the new technique of in vivo NIR spectroscopy can assess simultaneously both the immune response and graft function after liver transplantation.
H Ohdan; S Suzuki; M Kanashiro; H Amemiya; Y Fukuda; K Dohi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplant international : official journal of the European Society for Organ Transplantation     Volume:  7 Suppl 1     ISSN:  0934-0874     ISO Abbreviation:  Transpl. Int.     Publication Date:  1994  
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-04-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908516     Medline TA:  Transpl Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  S542-6     Citation Subset:  IM    
Second Department of Surgery, Hiroshima University, School of Medicine, Japan.
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MeSH Terms
Electron Transport Complex IV / analysis
Graft Rejection / diagnosis*,  pathology
Guanidines / therapeutic use
Hemoglobins / analysis*
Immunosuppressive Agents / therapeutic use
Liver Transplantation / pathology,  physiology*
Monitoring, Physiologic / methods
Nitrates / blood*
Nitric Oxide / metabolism*
Oxyhemoglobins / analysis
Rats, Inbred ACI
Rats, Inbred Lew
Spectrophotometry, Infrared / instrumentation,  methods
Transplantation, Homologous
Transplantation, Isogeneic
Reg. No./Substance:
0/Guanidines; 0/Hemoglobins; 0/Immunosuppressive Agents; 0/Nitrates; 0/Oxyhemoglobins; 0/nitrosyl hemoglobin; 10102-43-9/Nitric Oxide; 84937-45-1/gusperimus; EC Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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