Document Detail


In vivo molecular and cellular imaging with quantum dots.
MedLine Citation:
PMID:  15722017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Quantum dots (QDs), tiny light-emitting particles on the nanometer scale, are emerging as a new class of fluorescent probe for in vivo biomolecular and cellular imaging. In comparison with organic dyes and fluorescent proteins, QDs have unique optical and electronic properties: size-tunable light emission, improved signal brightness, resistance against photobleaching, and simultaneous excitation of multiple fluorescence colors. Recent advances have led to the development of multifunctional nanoparticle probes that are very bright and stable under complex in vivo conditions. A new structural design involves encapsulating luminescent QDs with amphiphilic block copolymers and linking the polymer coating to tumor-targeting ligands and drug delivery functionalities. Polymer-encapsulated QDs are essentially nontoxic to cells and animals, but their long-term in vivo toxicity and degradation need more careful study. Bioconjugated QDs have raised new possibilities for ultrasensitive and multiplexed imaging of molecular targets in living cells, animal models and possibly in humans.
Authors:
Xiaohu Gao; Lily Yang; John A Petros; Fray F Marshall; Jonathan W Simons; Shuming Nie
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Current opinion in biotechnology     Volume:  16     ISSN:  0958-1669     ISO Abbreviation:  Curr. Opin. Biotechnol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-21     Completed Date:  2005-06-24     Revised Date:  2013-07-02    
Medline Journal Info:
Nlm Unique ID:  9100492     Medline TA:  Curr Opin Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  63-72     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001, Atlanta, GA 30322, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Count / methods*
Cell Physiological Phenomena*
Cells
Humans
Image Enhancement / methods*
Microscopy, Fluorescence / methods*
Molecular Biology / methods
Molecular Probe Techniques*
Quantum Dots*
Spectrum Analysis / methods*
Grant Support
ID/Acronym/Agency:
P20 GM072069/GM/NIGMS NIH HHS; P20 GM072069/GM/NIGMS NIH HHS; R01 GM060562/GM/NIGMS NIH HHS; R01 GM60562/GM/NIGMS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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