| In vivo molecular and cellular imaging with quantum dots. | |
MedLine Citation:
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PMID: 15722017 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quantum dots (QDs), tiny light-emitting particles on the nanometer scale, are emerging as a new class of fluorescent probe for in vivo biomolecular and cellular imaging. In comparison with organic dyes and fluorescent proteins, QDs have unique optical and electronic properties: size-tunable light emission, improved signal brightness, resistance against photobleaching, and simultaneous excitation of multiple fluorescence colors. Recent advances have led to the development of multifunctional nanoparticle probes that are very bright and stable under complex in vivo conditions. A new structural design involves encapsulating luminescent QDs with amphiphilic block copolymers and linking the polymer coating to tumor-targeting ligands and drug delivery functionalities. Polymer-encapsulated QDs are essentially nontoxic to cells and animals, but their long-term in vivo toxicity and degradation need more careful study. Bioconjugated QDs have raised new possibilities for ultrasensitive and multiplexed imaging of molecular targets in living cells, animal models and possibly in humans. |
Authors:
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Xiaohu Gao; Lily Yang; John A Petros; Fray F Marshall; Jonathan W Simons; Shuming Nie |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review |
Journal Detail:
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Title: Current opinion in biotechnology Volume: 16 ISSN: 0958-1669 ISO Abbreviation: Curr. Opin. Biotechnol. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-02-21 Completed Date: 2005-06-24 Revised Date: 2013-07-02 |
Medline Journal Info:
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Nlm Unique ID: 9100492 Medline TA: Curr Opin Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 63-72 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001, Atlanta, GA 30322, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Count / methods* Cell Physiological Phenomena* Cells Humans Image Enhancement / methods* Microscopy, Fluorescence / methods* Molecular Biology / methods Molecular Probe Techniques* Quantum Dots* Spectrum Analysis / methods* |
| Grant Support | |
ID/Acronym/Agency:
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P20 GM072069/GM/NIGMS NIH HHS; P20 GM072069/GM/NIGMS NIH HHS; R01 GM060562/GM/NIGMS NIH HHS; R01 GM60562/GM/NIGMS NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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