Document Detail


In vivo microscopy in a porcine model of acute lung injury.
MedLine Citation:
PMID:  20638978     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Regional inhomogeneity and alveolar mechanics in a porcine model of acute lung injury (ALI) was evaluated using confocal laser scanning microscopy (CLSM). CLSM was performed through thoracic windows of the upper and lower lobes. Image quantification was conducted by use of a volume air index (VAI). Twelve anesthetized, mechanically ventilated pigs were randomized to non-injury (control group, n = 6) or ALI induced by surfactant depletion (ALI group, n = 6). CLSM was performed at baseline, after 1 h at 5 mbar and after 2 h at 15 mbar positive end-expiratory pressure (PEEP). Haemodynamics, respiratory mechanics and calculation of pulmonary ventilation-perfusion distribution by MIGET were determined. At baseline, VAI was not different. In the upper lobes, VAI significantly decreased in ALI compared to control group, with no changes after PEEP application. In the lower lobes, VAI significantly decreased in ALI compared to control group. Incremental PEEP significantly increased VAI in ALI, but not in control group. Haemodynamics were significantly compromised in the ALI group. A significant deterioration in oxygenation and ventilation-perfusion distribution could be seen being restored after PEEP adjustment. The VAI may help to assess regional inhomogeneity of the acutely injured lung.
Authors:
Johannes Bickenbach; Michael Czaplik; Rolf Dembinski; Paolo Pelosi; Wolfgang Schroeder; Gernot Marx; Rolf Rossaint
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Respiratory physiology & neurobiology     Volume:  172     ISSN:  1878-1519     ISO Abbreviation:  Respir Physiol Neurobiol     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-16     Completed Date:  2010-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101140022     Medline TA:  Respir Physiol Neurobiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  192-200     Citation Subset:  IM    
Affiliation:
Department of Intensive Care, University Hospital RWTH Aachen, Aachen, Germany. jbickenbach@ukaachen.de
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MeSH Terms
Descriptor/Qualifier:
Acute Lung Injury / pathology*
Animals
Body Temperature
Data Interpretation, Statistical
Female
Hemodynamics / physiology
Image Processing, Computer-Assisted
Microscopy, Confocal
Peak Expiratory Flow Rate / physiology
Pulmonary Alveoli / physiology
Pulmonary Artery / physiology
Pulmonary Circulation / physiology
Respiratory Mechanics / physiology
Swine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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