Document Detail


In vivo measurement and imaging of tumor oxygenation using coembedded paramagnetic particulates.
MedLine Citation:
PMID:  15334586     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tumor tissue oxygenation is an important parameter that is positively correlated to the chemo- or radiation treatment outcome of certain tumors. Hence, methods to accurately and noninvasively determine the concentration of oxygen (pO2) in tumors will be valuable. In this study, electron paramagnetic resonance (EPR) spectroscopy, utilizing microcrystalline particulates of lithium phthalocyanine (LiPc), was used to perform repeated measurements of pO2 as a function of tumor growth. We permanently embedded the particulates in the tumor by coimplanting them with RIF-1 tumor cells during inoculation in mice. This procedure enabled repeated measurements of oxygen concentration in the tumor to be obtained for >2 weeks during its growth phase. The particulates were stable and nontoxic to the tumor cells. Both an in vitro clonogenic assay and an in vivo tumor growth rate examination in C3H mice showed no apparent effect on cell proliferation or tumor growth rate. The measurements indicated that the pO2 of the tumor decreased exponentially with tumor growth and reached hypoxic levels ( approximately 4 mmHg) within 4 days after inoculation of the tumor cells. Spatial EPR imaging revealed a nonuniform distribution of the embedded particulates, which were localized mainly in the middle of the tumor volume. Oxygen mapping of the tumor, obtained by spectroscopic EPR imaging, showed significant variation of pO2 within the tumor. In summary, EPR spectroscopy and imaging with an embedded oximetry probe enabled accurate and repeated measurements of pO2 to be obtained in growing tumors under nonperturbing conditions.
Authors:
Govindasamy Ilangovan; Anna Bratasz; Haiquan Li; Petra Schmalbrock; Jay L Zweier; Periannan Kuppusamy
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  52     ISSN:  0740-3194     ISO Abbreviation:  Magn Reson Med     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-08-30     Completed Date:  2004-12-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  650-7     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Wiley-Liss, Inc.
Affiliation:
Center for Biomedical EPR Spectroscopy and Imaging, Davis Heart and Lung Research Institute, Department of Internal Medicine, Ohio State University, Columbus, USA.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Electron Spin Resonance Spectroscopy / methods*
Fibrosarcoma / metabolism*,  pathology
Indoles / diagnostic use*
Mice
Mice, Inbred C3H
Neoplasm Transplantation
Organometallic Compounds / diagnostic use*
Oxygen / metabolism*
Grant Support
ID/Acronym/Agency:
CA 78886/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Indoles; 0/Organometallic Compounds; 111716-29-1/lithium phthalocyanine; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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