Document Detail

In vivo measurement of dopamine and its metabolites by intracerebral dialysis: changes after d-amphetamine.
MedLine Citation:
PMID:  6196446     Owner:  NLM     Status:  MEDLINE    
By using a new technique, intracerebral dialysis, in combination with high performance liquid chromatography and electrochemical detection, it was possible to recover and measure endogenous extracellular dopamine, together with its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) from the striatum and nucleus accumbens of anaesthetized or freely moving rats. In addition, measurements of extracellular 5-hydroxyindoleacetic acid, ascorbic acid, and uric acid were made. Basal extracellular concentrations of dopamine and DOPAC in the striatum were estimated to be 5 X 10(-8) M and 5 X 10(-6) M, respectively. d-Amphetamine (2 mg/kg s.c.) increased dopamine levels in the striatum perfusates by 14-fold, whereas levels of DOPAC and HVA decreased by 77% and 66%, respectively.
T Zetterström; T Sharp; C A Marsden; U Ungerstedt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  41     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1769-73     Citation Subset:  IM    
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / analysis
Ascorbic Acid / analysis
Brain / drug effects
Brain Chemistry*
Dextroamphetamine / pharmacology*
Dialysis / methods
Dopamine / analysis*
Homovanillic Acid / analysis
Hydroxyindoleacetic Acid / analysis
Rats, Inbred Strains
Uric Acid / analysis
Reg. No./Substance:
102-32-9/3,4-Dihydroxyphenylacetic Acid; 306-08-1/Homovanillic Acid; 50-81-7/Ascorbic Acid; 51-64-9/Dextroamphetamine; 54-16-0/Hydroxyindoleacetic Acid; 69-93-2/Uric Acid

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