Document Detail


In vivo magnetic resonance imaging of injected mesenchymal stem cells in rat myocardial infarction; simultaneous cell tracking and left ventricular function measurement.
MedLine Citation:
PMID:  19132547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine whether magnetic resonance imaging (MRI) can enable magnetically labeled mesenchymal stem cell (MSC) tracking and simultaneous in vivo functional data acquisition in rat models of myocardial infarction. Superparamagnetic iron oxide-laden human MSCs were injected into rat myocardium infarcted by cryoinjury 3 weeks after myocardial infarction. The control group received cell-free media injection. Before injection and for 3 months after, in vivo serial MRI was performed. Electrocardiography-gated gradient echo sequence MRI and cine MRI were performed for in vivo cell tracking and assessing cardiac function using left ventricular ejection fraction (LVEF), respectively. MRI revealed a persistent signal-void representing iron-laden MSCs until ten post-injection weeks. Serial follow-up MRI revealed that LVEF was significantly higher in the MSC injection group than in the control group. We conclude that MRI enables in vivo tracking of injected cells and evaluation of the long-term therapeutic potential of MSCs for myocardial infarction.
Authors:
Young Jin Kim; Yong-Min Huh; Kyu Ok Choe; Byoung Wook Choi; Eun Jeong Choi; Yangsoo Jang; Jae Myun Lee; Jin-Suck Suh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-09
Journal Detail:
Title:  The international journal of cardiovascular imaging     Volume:  25 Suppl 1     ISSN:  1875-8312     ISO Abbreviation:  Int J Cardiovasc Imaging     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-24     Completed Date:  2009-07-09     Revised Date:  2013-04-11    
Medline Journal Info:
Nlm Unique ID:  100969716     Medline TA:  Int J Cardiovasc Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  99-109     Citation Subset:  IM    
Affiliation:
Department of Radiology and Research Institute of Radiological Science, Severance Hospital, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, 120-752 Seoul, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement*
Cells, Cultured
Contrast Media / diagnostic use
Dextrans
Disease Models, Animal
Electrocardiography
Ferumoxytol
Humans
Iron / diagnostic use
Magnetic Resonance Imaging, Cine*
Magnetite Nanoparticles
Male
Mesenchymal Stem Cell Transplantation*
Myocardial Infarction / pathology,  physiopathology,  surgery*
Myocardium / pathology*
Oxides / diagnostic use
Rats
Rats, Sprague-Dawley
Stroke Volume
Time Factors
Ventricular Function, Left*
Chemical
Reg. No./Substance:
0/Contrast Media; 0/Magnetite Nanoparticles; 0/Oxides; 1317-61-9/Ferumoxytol; 7439-89-6/Iron; 9004-54-0/Dextrans; G6N3J05W84/ferumoxides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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