Document Detail

In vivo on-line HPLC-microdialysis: simultaneous detection of monoamines and their metabolites in awake freely-moving rats.
MedLine Citation:
PMID:  10704106     Owner:  NLM     Status:  MEDLINE    
An on-line microbore high performance liquid chromatographic--electrochemical (HPLC--EC) detection method has been developed for the simultaneous measurement of dopamine (3,4-dihydroxyphenethylamine, DA), its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (3-methoxy-4-hydroxyphenylacetic acid, HVA), as well as serotonin (5-hydroxytryptamine, 5-HT) and its metabolise 5-hydroxyindole-3-acetic acid (5-HIAA) in the striatum of awake and freely moving rats. Our method was capable of detecting DOPAC, 5-HIAA, HVA, DA and 5-HT at retention time of 3, 5, 6.5, 9, and 24 min, respectively, in repeated on-line microdialysis sampling. Analysis was performed using a 150 x 1 mm 5 microm C18 microbore column attached directly to a thin-layer radial flow electrochemical cell (UniJet) comprising a 6 mm glassy carbon working electrode. In order to accommodate signal outputs (due to a varying level in the neurochemicals under investigation), an amperometric detector was equipped with a sensitivity programmable controlling software for automatically switching sensitivity inattentively and repeatedly for each collection cycle.
C S Chaurasia; C E Chen; C R Ashby
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  19     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  1999 Mar 
Date Detail:
Created Date:  2000-03-17     Completed Date:  2000-03-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  413-22     Citation Subset:  IM    
Department of Pharmacy and Administrative Sciences, College of Pharmacy and Allied Health Professions, St. John's University, Jamaica, New York 11439, USA.
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / analysis,  metabolism
Acetonitriles / chemistry
Biogenic Monoamines / analysis*,  chemistry,  metabolism
Chromatography, High Pressure Liquid / instrumentation,  methods
Corpus Striatum / metabolism
Dopamine / analysis,  metabolism
Homovanillic Acid / analysis,  metabolism
Hydroxyindoleacetic Acid / analysis,  metabolism
Microdialysis / instrumentation,  methods*
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Serotonin / analysis,  metabolism
Reg. No./Substance:
0/Acetonitriles; 0/Biogenic Monoamines; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 306-08-1/Homovanillic Acid; 50-67-9/Serotonin; 54-16-0/Hydroxyindoleacetic Acid; 75-05-8/acetonitrile

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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