Document Detail

In vivo influence of the anti-B220 monoclonal antibody administration of the T cell differentiation in mice.
MedLine Citation:
PMID:  2475748     Owner:  NLM     Status:  MEDLINE    
B220, a pan-B marker, is known to be also expressed on immature T cells of MRL/1pr or other congenic 1pr mice and minor population of immature thymocytes but not on peripheral T cells. In this study, we investigated in vivo the possibility whether B220 is one kind of premature T or prethymic T precursor cell marker as well as a pan-B cell marker. A monoclonal antibody against B220 glycoprotein was intravenously injected every 2 days into various strains of mice. After the third administration of this antibody, thymocytes decreased remarkably compared with those from the rat IgG-treated group, and spleen cells were also reduced significantly. Further, the number of cells expressing Thy-1, Ly-1, Lyt-2, and asialo GM1 (asGM1) in the spleen were significantly reduced. On the contrary, the number of cells expressing surface IgM (sIgM) or B220 were increased by this treatment, especially after the 8th treatment. Some T-dependent immunological functions including mitogenic responses to lectins and cytotoxic T cell activity were markedly suppressed but mixed lymphocyte reaction (MLR) and natural killer (NK) activity were rather augmented. Thus, B220 may be expressed on some kinds of T cell progenitor. Taken together, in vivo treatment with anti-B220 antibody may influence differentiating stages of some T cells from bone marrow progenitors before or just after their homing into the thymus.
V Assensi; K Himeno; I Kawamura; M Sakumoto; K Nomoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbiology and immunology     Volume:  33     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  1989  
Date Detail:
Created Date:  1989-10-05     Completed Date:  1989-10-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  329-39     Citation Subset:  IM    
Department of Immunology, Kyushu University, Fukuoka.
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MeSH Terms
Antibodies, Monoclonal / administration & dosage*
Antigens, CD45
Antigens, Differentiation, T-Lymphocyte / immunology*
Cell Differentiation / drug effects
Cytotoxicity Tests, Immunologic
Dose-Response Relationship, Immunologic
Fluorescent Antibody Technique
Hematopoietic Stem Cells / immunology
Killer Cells, Natural / immunology
Mice, Inbred BALB C
Mitogens / pharmacology
Spleen / cytology,  drug effects
T-Lymphocytes / cytology*,  immunology
Thymus Gland / cytology,  drug effects
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Differentiation, T-Lymphocyte; 0/Mitogens; EC, CD45

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