Document Detail

In vivo induction of functional Fc gammaRI (CD64) on neutrophils and modulation of blood cytokine mRNA levels in cancer patients treated with G-CSF (rMetHuG-CSF).
MedLine Citation:
PMID:  9504638     Owner:  NLM     Status:  MEDLINE    
Neutrophils from 13 children who received G-CSF for the collection of peripheral blood progenitors while they were in haematological steady state were studied at various times after G-CSF injection for Fc gammaR expression (Fc gammaRI or CD64, Fc gammaRII or CD32, and Fc gammaRIII or CD16) and for their ability to exert antibody-dependent cell cytotoxicity (ADCC) through Fc gammaRI. Changes in IFNgamma, IL8, IL10, MCP1 and TNF alpha mRNA levels in peripheral blood cells were also studied 4 h and 24 h after the first G-CSF injection. Fc gammaRI expression increased strongly after 24 h and then remained at the same level throughout treatment. In contrast, Fc gammaRIII expression sharply decreased at day 1 and diminished even further thereafter. No change in Fc gammaRII was observed. ADCC exerted by neutrophils through Fc gammaRI started to increase after 24 h with the peak level at day 5. Cytokine mRNA analyses indicated a reproducible and strong increase of IL8 mRNA (11/13 children) after 24 h, whereas the changes in the mRNA levels of the other cytokines tested were more heterogenous (TFNgamma: three; IL10: six; MCP1: five: TNF alpha: four, of the 13 children). Therefore this study opens the way to an optimized therapeutic schedule for the combined use of G-CSF and monoclonal antibodies in adjuvant immuno-intervention.
J M Michon; A Gey; S Moutel; E Tartour; V Meresse; W Fridman; J L Teillaud
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of haematology     Volume:  100     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-03-27     Completed Date:  1998-03-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  550-6     Citation Subset:  IM    
INSERM Unité 255, Institut Curie, Paris, France.
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MeSH Terms
Antibody-Dependent Cell Cytotoxicity / immunology*
Child, Preschool
Cytokines / genetics,  metabolism*
Filgrastim / therapeutic use*
Hematopoietic Stem Cell Mobilization
Interleukin-8 / pharmacology
Leukocyte Count
Neoplasms / drug therapy,  immunology*
Neutrophils / immunology*
RNA, Messenger / metabolism
Receptors, IgG / immunology*
Reg. No./Substance:
0/Cytokines; 0/Interleukin-8; 0/RNA, Messenger; 0/Receptors, IgG; 121181-53-1/Filgrastim

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