| In vivo-induced InvA-like autotransporters Ifp and InvC of Yersinia pseudotuberculosis promote interactions with intestinal epithelial cells and contribute to virulence. | |
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MedLine Citation:
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PMID: 22158741 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The Yersinia pseudotuberculosis Ifp and InvC molecules are putative autotransporter proteins with a high homology to the invasin (InvA) protein. In order to characterize the function of these surface proteins we expressed both factors in Escherichia coli K-12 and demonstrated attachment of Ifp- and InvC-expressing bacteria to human-, mouse- and pig-derived intestinal epithelial cells. Ifp was also found to mediate microcolony formation and internalization into polarized human enterocytes. The ifp and invC genes were not expressed under in vitro conditions, but were found to be induced in the Peyer's patches of the mouse intestinal tract. In a murine co-infection model, colonization of the Peyer's patches and the mesenteric lymph nodes of mice by the ifp deficient strain was significantly reduced and considerably fewer bacteria reached liver and spleen. Absence of InvC had no severe influence on bacterial colonization in the murine infection model, and resulted only in a slightly reduced number of invC mutants in the Peyer's patches. Analysis of the host immune response demonstrated that presence of Ifp and InvC reduced the recruitment of professional phagocytes, especially neutrophils, in the Peyer's patches. These findings support a role for the adhesins in modulating host-pathogen interactions important for the immune defense. |
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Authors:
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Fabio Pisano; Annika Kochut; Frank Uliczka; Rebecca Geyer; Tatjana Stolz; Tanja Thiermann; Manfred Rohde; Petra Dersch |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-12 |
Journal Detail:
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Title: Infection and immunity Volume: - ISSN: 1098-5522 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Abteilung Molekulare Infektionsbiologie, Helmholtz-Zentrum für Infektionsforschung, 38124 Braunschweig, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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