Document Detail


In vivo and in vitro growth stimulation of murine hepatoma cells by glucocorticoid.
MedLine Citation:
PMID:  12168818     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An increased level of glucocorticoid receptors has been found in hepatoma. The aim of this study was to determine the in vivo effect of glucocorticoid on the growth of murine hepatoma cells. In vitro cell growth was determined by MTT assay, while cell cycle progression was assayed with flow cytometry. For in vivo experiments, ML-3 and Hepa 1-6 cells were implanted in BALB/c and SCID mice, respectively. Each mouse received 4 subcutaneous injections of hydrocortisone and/or RU486 after tumor implantation. We found that glucocorticoid treatment of ML-3 and Hepa 1-6 cells in vitro resulted in an increase in cell growth which was partially inhibited by RU486, a glucocorticoid antagonist. Glucocorticoid treatment enhanced the cell cycle progression of ML-3 cells. The incidence of ML-3 tumor in the hydrocortisone group was significantly higher than that of the saline, RU486, or hydrocortisone plus RU486 groups. RU486 partially blocked the hydrocortisone-stimulated growth of hepatoma. Further study showed that glucocorticoid treatment of SCID mice also stimulated Hepa 1-6 tumor growth. In conclusion, our results indicated that glucocorticoid directly stimulated hepatoma growth and this stimulation was not the result of immune suppression. Glucocorticoids may play an important role in regulating the growth of hepatoma.
Authors:
Wing-Yiu Lui; Chin-Wen Chi; Yuh-Fang Chang; Hoi-Weng Chu; Chih-Chang Hsieh; Pen-Hui Yin; Tsung-Yun Liu; Ying-Ru Ou; Fang-Ku P'eng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  22     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2002 May-Jun
Date Detail:
Created Date:  2002-08-09     Completed Date:  2002-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1413-22     Citation Subset:  IM    
Affiliation:
Department of Surgery, Veterans General Hospital-Taipei, Taiwan, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects
Dexamethasone / pharmacology*
Glucocorticoids / physiology
Growth Substances / pharmacology
Hormone Antagonists / pharmacology
Hydrocortisone / pharmacology*
Liver Neoplasms, Experimental / metabolism,  pathology*
Male
Mice
Mice, Inbred BALB C
Mice, SCID
Mifepristone / pharmacology
Receptors, Glucocorticoid / metabolism,  physiology
Chemical
Reg. No./Substance:
0/Glucocorticoids; 0/Growth Substances; 0/Hormone Antagonists; 0/Receptors, Glucocorticoid; 50-02-2/Dexamethasone; 50-23-7/Hydrocortisone; 84371-65-3/Mifepristone

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