Document Detail

In vivo and in vitro evidence for autocrine DCoH/HNF-1alpha transcription of albumin in the human epidermis.
MedLine Citation:
PMID:  15740590     Owner:  NLM     Status:  MEDLINE    
The presence of albumin in the human epidermis has been reported more than a decade ago, but until now, it was assumed that this protein is synthesized in the liver and transported to the avascular skin. To our knowledge, transcription of albumin in the human epidermis was never considered. In this report, we present for the first time evidence for autocrine synthesis of albumin in the human epidermis in keratinocytes in situ and in vitro. Using double immunofluorescence labelling, we identified that albumin colocalized together with its transcription factor PCD/DCoH/HNF-1alpha in suprabasal keratinocytes in human full-thickness skin sections and in keratinocytes cultured in serum-free medium. Moreover, albumin and HNF-1alpha protein expression was confirmed by Western blotting in undifferentiated and differentiated keratinocytes as well as in human epidermal suction blister roof extracts. Reverse-transcriptase polymerase chain reaction analysis from human epidermal keratinocytes and epidermal suction blister roofs revealed the transcription of albumin. Using in vivo fluorescence excitation spectroscopy at the surface of human skin, we confirmed albumin as a major constituent yielding a lambda(max) at 295 nm, which was assigned to the single tryptophan 214 fluorophore in this protein. This in vivo result is in agreement with albumin concentrations of 10(-3) M, underlining the importance of this protein in epidermal homeostasis.
S Hasse; S Kothari; H Rokos; S Kauser; N Y Schürer; K U Schallreuter
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental dermatology     Volume:  14     ISSN:  0906-6705     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-02     Completed Date:  2005-08-09     Revised Date:  2008-05-14    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  182-7     Citation Subset:  IM    
Clinical and Experimental Dermatology, Department of Biomedical Sciences, University of Bradford, Bradford, West Yorkshire BD7 1DP, UK.
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MeSH Terms
Albumins / genetics*
Autocrine Communication / physiology*
Blister / metabolism
Blotting, Western
Cells, Cultured
DNA-Binding Proteins / metabolism*
Epidermis / metabolism*
Hepatocyte Nuclear Factor 1
Hepatocyte Nuclear Factor 1-alpha
Hydro-Lyases / metabolism*
Keratinocytes / metabolism
Nuclear Proteins / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Spectrometry, Fluorescence
Transcription Factors / metabolism*
Transcription, Genetic / physiology*
Reg. No./Substance:
0/Albumins; 0/DNA-Binding Proteins; 0/HNF1A protein, human; 0/Hepatocyte Nuclear Factor 1-alpha; 0/Nuclear Proteins; 0/Transcription Factors; 126548-29-6/Hepatocyte Nuclear Factor 1; EC 4.2.1.-/Hydro-Lyases; EC dehydratase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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