Document Detail


In vivo and in vitro anti-inflammatory activities of alpha-linolenic acid isolated from Actinidia polygama fruits.
MedLine Citation:
PMID:  17679548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fruit of Actinidia polygama (AP) has long been used as a folk medicine in Korea for the treatment of pain, rheumatoid arthritis and inflammation. In the present study, bioassay-guided fractionation of AP led to the separation and identification of a polyunsaturated fatty acid, alpha-linolenic acid (ALA), which was found to show anti-inflammatory activity. The anti-inflammatory effects of ALA, using acetic acid or carrageenan-induced inflammation models, were investi gated in mice or rats, respectively. ALA significantly inhibited the acetic acid-induced vascular permeability in a dose dependent manner (34.2 and 37.7% inhibition at doses of 5 and 10 mg/ kg, respectively). ALA also significantly reduced a rat paw edema induced by a single treatment of carrageenan. To investigate the mechanism of the anti-inflammatory action of ALA, the effects of ALA on lipopolysaccharide (LPS)-induced responses in the murine mac rophages cell line, RAW 264.7, were examined. Exposure of LPS-stimulated cells to ALA inhibited the accumulation of nitrite and prostaglandin E2 (PGE2) in the culture medium. Consistent with these observations, the protein and mRNA expression levels of iNOS and COX-2 enzyme were markedly inhibited by ALA in a dose dependent manner. These results suggest that the anti-inflammatory activity of ALA might be due to the suppression of the expressions of iNOS and COX-2 mRNA.
Authors:
Jie Ren; Eun Jung Han; Sung Hyun Chung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of pharmacal research     Volume:  30     ISSN:  0253-6269     ISO Abbreviation:  Arch. Pharm. Res.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-08-07     Completed Date:  2007-10-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000036     Medline TA:  Arch Pharm Res     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  708-14     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Clinical Pharmacy, College of Pharmacy, Kyung Hee University, Dongdaemun-gu, Seoul 130-701, Korea.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid
Actinidia / chemistry*
Aminolevulinic Acid / metabolism
Animals
Anti-Inflammatory Agents / isolation & purification*,  pharmacology*
Blotting, Western
Capillary Permeability / drug effects
Carrageenan
Cell Line
Cell Survival / drug effects
Cyclooxygenase 2 / metabolism
Cyclooxygenase 2 Inhibitors / pharmacology
Dinoprostone / analysis,  biosynthesis
Edema / chemically induced,  prevention & control
Enzyme Inhibitors / pharmacology
Fruit / chemistry*
Hexanes
Indicators and Reagents
Lipopolysaccharides / pharmacology
Macrophages / drug effects
Male
Mice
Mice, Inbred ICR
Nitric Oxide Synthase Type II / antagonists & inhibitors,  biosynthesis
Plant Extracts / chemistry
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Solvents
Tetrazolium Salts
Thiazoles
alpha-Linolenic Acid / isolation & purification*,  pharmacology*
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Cyclooxygenase 2 Inhibitors; 0/Enzyme Inhibitors; 0/Hexanes; 0/Indicators and Reagents; 0/Lipopolysaccharides; 0/Plant Extracts; 0/Solvents; 0/Tetrazolium Salts; 0/Thiazoles; 106-60-5/Aminolevulinic Acid; 298-93-1/thiazolyl blue; 363-24-6/Dinoprostone; 463-40-1/alpha-Linolenic Acid; 64-19-7/Acetic Acid; 9000-07-1/Carrageenan; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.99.1/Cyclooxygenase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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