Document Detail


In vivo and in vitro analysis of the vasculogenic potential of avian proepicardial and epicardial cells.
MedLine Citation:
PMID:  16456846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Coronary vessel formation is a special case in the context of embryonic vascular development. A major part of the coronary cellular precursors (endothelial, smooth muscle, and fibroblastic cells) derive from the proepicardium and the epicardium in what can be regarded as a late event of angioblastic and smooth muscle cell differentiation. Thus, coronary morphogenesis is dependent on the epithelial-mesenchymal transformation of the proepicardium and the epicardium. In this study, we present several novel observations about the process of coronary vasculogenesis in avian embryos, namely: (1) The proepicardium displays a high vasculogenic potential, both in vivo (as shown by heterotopic transplants) and in vitro, which is modulated by vascular endothelial growth factor (VEGF) and basic fibroblast growth factor signals; (2) Proepicardial and epicardial cells co-express receptors for platelet-derived growth factor-BB and VEGF; (3) Coronary angioblasts (found all through the epicardial, subepicardial, and compact myocardial layers) express the Wilms' tumor associated transcription factor and the retinoic acid-synthesizing enzyme retinaldehyde-dehydrogenase-2, two markers of the coelomic epithelium involved in coronary endothelium development. All these results contribute to the development of our knowledge on the vascular potential of proepicardial/epicardial cells, the existent interrelationships between the differentiating coronary cell lineages, and the molecular mechanisms involved in the regulation of coronary morphogenesis.
Authors:
Juan A Guadix; Rita Carmona; Ramón Muñoz-Chápuli; José M Pérez-Pomares
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  235     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-20     Completed Date:  2006-06-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1014-26     Citation Subset:  IM    
Copyright Information:
(c) 2006 Wiley-Liss, Inc.
Affiliation:
Department of Animal Biology, Faculty of Science, University of Málaga, Campus de Teatinos s/n, Málaga 29071, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques
Cell Differentiation
Cell Lineage
Cells, Cultured
Chick Embryo
Chimera
Collagen Type I / genetics,  metabolism
Coronary Vessels / cytology*,  embryology,  metabolism
Coturnix
Endothelium, Vascular / cytology*,  metabolism
Fibroblast Growth Factor 2 / metabolism,  pharmacology
Fibroblasts / cytology,  metabolism
Gels
Heart / embryology*
Immunohistochemistry
Mesoderm / cytology,  metabolism
Microscopy, Confocal
Muscle, Smooth, Vascular / cytology*,  embryology,  metabolism
Organ Culture Techniques
Pericardium / cytology*,  embryology,  metabolism
Rats
Transplantation, Heterotopic
Vascular Endothelial Growth Factor A / metabolism,  pharmacology
Grant Support
ID/Acronym/Agency:
N01 HD 23144/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Collagen Type I; 0/Gels; 0/Vascular Endothelial Growth Factor A; 103107-01-3/Fibroblast Growth Factor 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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