| In vivo and in vitro analysis of the vasculogenic potential of avian proepicardial and epicardial cells. | |
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MedLine Citation:
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PMID: 16456846 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Coronary vessel formation is a special case in the context of embryonic vascular development. A major part of the coronary cellular precursors (endothelial, smooth muscle, and fibroblastic cells) derive from the proepicardium and the epicardium in what can be regarded as a late event of angioblastic and smooth muscle cell differentiation. Thus, coronary morphogenesis is dependent on the epithelial-mesenchymal transformation of the proepicardium and the epicardium. In this study, we present several novel observations about the process of coronary vasculogenesis in avian embryos, namely: (1) The proepicardium displays a high vasculogenic potential, both in vivo (as shown by heterotopic transplants) and in vitro, which is modulated by vascular endothelial growth factor (VEGF) and basic fibroblast growth factor signals; (2) Proepicardial and epicardial cells co-express receptors for platelet-derived growth factor-BB and VEGF; (3) Coronary angioblasts (found all through the epicardial, subepicardial, and compact myocardial layers) express the Wilms' tumor associated transcription factor and the retinoic acid-synthesizing enzyme retinaldehyde-dehydrogenase-2, two markers of the coelomic epithelium involved in coronary endothelium development. All these results contribute to the development of our knowledge on the vascular potential of proepicardial/epicardial cells, the existent interrelationships between the differentiating coronary cell lineages, and the molecular mechanisms involved in the regulation of coronary morphogenesis. |
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Authors:
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Juan A Guadix; Rita Carmona; Ramón Muñoz-Chápuli; José M Pérez-Pomares |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 235 ISSN: 1058-8388 ISO Abbreviation: Dev. Dyn. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-20 Completed Date: 2006-06-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 1014-26 Citation Subset: IM |
Copyright Information:
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(c) 2006 Wiley-Liss, Inc. |
Affiliation:
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Department of Animal Biology, Faculty of Science, University of Málaga, Campus de Teatinos s/n, Málaga 29071, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques Cell Differentiation Cell Lineage Cells, Cultured Chick Embryo Chimera Collagen Type I / genetics, metabolism Coronary Vessels / cytology*, embryology, metabolism Coturnix Endothelium, Vascular / cytology*, metabolism Fibroblast Growth Factor 2 / metabolism, pharmacology Fibroblasts / cytology, metabolism Gels Heart / embryology* Immunohistochemistry Mesoderm / cytology, metabolism Microscopy, Confocal Muscle, Smooth, Vascular / cytology*, embryology, metabolism Organ Culture Techniques Pericardium / cytology*, embryology, metabolism Rats Transplantation, Heterotopic Vascular Endothelial Growth Factor A / metabolism, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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N01 HD 23144/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I; 0/Gels; 0/Vascular Endothelial Growth Factor A; 103107-01-3/Fibroblast Growth Factor 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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