Document Detail


In vivo imaging of mucosal CD4+ T cells using single photon emission computed tomography in a murine model of colitis.
MedLine Citation:
PMID:  17964595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immune responses that occur in the context of human infectious and inflammatory diseases are usually studied by sampling cells from peripheral blood, from biopsies, or by end-point harvests at necropsy. These approaches are likely to yield information that is incomplete and/or non-representative. Here, we report the development and validation of a non-invasive method to localize and to quantitate the disposition of specific subpopulations of cells in vivo. In a murine model of dextran sulfate sodium (DSS)-induced colitis, CD4+ T cells were visualized in the colon by single photon emission computed tomography (SPECT-CT) after injection of monoclonal, non-depleting, indium-111 (111In) labeled anti-CD4+ antibodies. The SPECT-CT colon uptake ratio (CUR) was found to correlate (p<0.01) with the number of total CD4+ T cells and with standard measures of pathology (colon length, cell counts, and histopathologic evidence of apoptosis, edema, and cellular infiltrates) as assessed by direct examination of diseased colon. Each of these parameters, including the SPECT-CT signal uptake, increased as a function of DSS dose (p<0.05). We conclude that CT-SPECT imaging using an 111In-labeled anti-CD4+ antibody is reflective of traditional parameters of pathology in this experimental model of murine colitis. This approach should be readily applicable to the imaging of discrete cell subpopulations in non-human primates and in humans, thus augmenting our understanding of infectious diseases and inflammation in vivo.
Authors:
Bittoo Kanwar; Dong Wei Gao; Andrew B Hwang; James P Grenert; Simon P Williams; Benjamin Franc; Joseph M McCune
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2007-10-16
Journal Detail:
Title:  Journal of immunological methods     Volume:  329     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-17     Completed Date:  2008-03-20     Revised Date:  2014-06-12    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  21-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use
CD4-Positive T-Lymphocytes / immunology,  radionuclide imaging*
Colitis / chemically induced,  immunology,  pathology,  radionuclide imaging*
Colon / immunology,  pathology,  radionuclide imaging*
Dextran Sulfate
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Indium Radioisotopes / diagnostic use
Intestinal Mucosa / immunology,  pathology,  radionuclide imaging*
Mice
Mice, Inbred BALB C
Rats
Reproducibility of Results
Severity of Illness Index
Tomography, Emission-Computed, Single-Photon / methods*
Grant Support
ID/Acronym/Agency:
0D00329//PHS HHS; DP1 OD000329/OD/NIH HHS; DP1 OD000329-01/OD/NIH HHS; DP1 OD000329-02/OD/NIH HHS; DP1 OD000329-03/OD/NIH HHS; DP1 OD000329-04/OD/NIH HHS; K24 DK060617/DK/NIDDK NIH HHS; R37 AI040312/AI/NIAID NIH HHS; R37 AI040312-09/AI/NIAID NIH HHS; R37 AI040312-10/AI/NIAID NIH HHS; R37 AI040312-11/AI/NIAID NIH HHS; R37 AI040312-12/AI/NIAID NIH HHS; R37 AI40312/AI/NIAID NIH HHS; T32 DK007762/DK/NIDDK NIH HHS; T32-007762//PHS HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Indium Radioisotopes; 9042-14-2/Dextran Sulfate
Comments/Corrections

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