Document Detail

In vivo imaging of embryonic vascular development using transgenic zebrafish.
MedLine Citation:
PMID:  12167406     Owner:  NLM     Status:  MEDLINE    
In this study we describe a model system that allows continuous in vivo observation of the vertebrate embryonic vasculature. We find that the zebrafish fli1 promoter is able to drive expression of enhanced green fluorescent protein (EGFP) in all blood vessels throughout embryogenesis. We demonstrate the utility of vascular-specific transgenic zebrafish in conjunction with time-lapse multiphoton laser scanning microscopy by directly observing angiogenesis within the brain of developing embryos. Our images reveal that blood vessels undergoing active angiogenic growth display extensive filopodial activity and pathfinding behavior similar to that of neuronal growth cones. We further show, using the zebrafish mindbomb mutant as an example, that the expression of EGFP within developing blood vessels permits detailed analysis of vascular defects associated with genetic mutations. Thus, these transgenic lines allow detailed analysis of both wild type and mutant embryonic vasculature and, together with the ability to perform large scale forward-genetic screens in zebrafish, will facilitate identification of new mutants affecting vascular development.
Nathan D Lawson; Brant M Weinstein
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  248     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-08     Completed Date:  2002-09-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  307-18     Citation Subset:  IM    
Laboratory of Molecular Genetics, NICHD/NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA.
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MeSH Terms
Animals, Genetically Modified
Blood Vessels / embryology*
Brain / blood supply*,  embryology*
Genes, Reporter / genetics
Germ-Line Mutation / genetics
Green Fluorescent Proteins
Luminescent Proteins / genetics,  metabolism
Microscopy, Confocal / methods*
Promoter Regions, Genetic / genetics
Time Factors
Transgenes / genetics*
Zebrafish / embryology*,  genetics*
Zebrafish Proteins / genetics,  metabolism
Reg. No./Substance:
0/Luminescent Proteins; 0/Zebrafish Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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