Document Detail


In vivo imaging of embryonic vascular development using transgenic zebrafish.
MedLine Citation:
PMID:  12167406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we describe a model system that allows continuous in vivo observation of the vertebrate embryonic vasculature. We find that the zebrafish fli1 promoter is able to drive expression of enhanced green fluorescent protein (EGFP) in all blood vessels throughout embryogenesis. We demonstrate the utility of vascular-specific transgenic zebrafish in conjunction with time-lapse multiphoton laser scanning microscopy by directly observing angiogenesis within the brain of developing embryos. Our images reveal that blood vessels undergoing active angiogenic growth display extensive filopodial activity and pathfinding behavior similar to that of neuronal growth cones. We further show, using the zebrafish mindbomb mutant as an example, that the expression of EGFP within developing blood vessels permits detailed analysis of vascular defects associated with genetic mutations. Thus, these transgenic lines allow detailed analysis of both wild type and mutant embryonic vasculature and, together with the ability to perform large scale forward-genetic screens in zebrafish, will facilitate identification of new mutants affecting vascular development.
Authors:
Nathan D Lawson; Brant M Weinstein
Related Documents :
24246936 - Factors that influence mercury concentrations in nestling eagle owls (bubo bubo).
20639516 - Wood anatomical analysis of alnus incana and betula pendula injured by a debris-flow ev...
22359646 - S phase entry of neural progenitor cells correlates with increased blood flow in the yo...
12004336 - The blood supply of the growth plate and the epiphysis: a comparative scanning electron...
6604926 - The ramp injection of radiotracers for blood flow measurement by emission tomography.
8553296 - Diagnostic value of blood cytokine concentrations in acute pneumonia.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  248     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-08     Completed Date:  2002-09-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  307-18     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Genetics, NICHD/NIH, Building 6B, 6 Center Drive, Bethesda, MD 20892, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Blood Vessels / embryology*
Brain / blood supply*,  embryology*
Genes, Reporter / genetics
Germ-Line Mutation / genetics
Green Fluorescent Proteins
Luminescent Proteins / genetics,  metabolism
Microscopy, Confocal / methods*
Promoter Regions, Genetic / genetics
Time Factors
Transgenes / genetics*
Zebrafish / embryology*,  genetics*
Zebrafish Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 0/Zebrafish Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Terminal glial differentiation involves regulated expression of the excitatory amino acid transporte...
Next Document:  Development of dorsal-ventral polarity in the optic vesicle and its presumptive role in eye morphoge...