Document Detail

In vivo imaging of dopaminergic neurotransmission after transient focal ischemia in rats.
MedLine Citation:
PMID:  23149560     Owner:  NLM     Status:  MEDLINE    
The precise biologic mechanisms involved in functional recovery processes in response to stroke such as dopaminergic neurotransmission are still largely unknown. For this purpose, we performed in parallel in vivo magnetic resonance imaging and positron emission tomography (PET) with [(18)F]fluorodeoxyglucose ([(18)F]FDG) and [(11)C]raclopride at 1, 3, 7, 14, 21, and 28 days after middle cerebral artery occlusion in rats. In the ischemic territory, PET [(18)F]FDG showed a initial decrease in cerebral metabolism followed by a time-dependent recovery to quasi-normal values at day 14 after ischemia. The PET with [(11)C]raclopride, a ligand for dopamine D(2) receptor, showed a sustained binding during the first week after ischemia that declined dramatically from day 14 to day 28. Interestingly, a slight increase in [(11)C]raclopride binding was observed at days 1 to 3 followed by the uppermost binding at day 7 in the contralateral territory. Likewise, in vitro autoradiography using [(3)H]raclopride confirmed these in vivo results. Finally, the neurologic test showed major neurologic impairment at day 1 followed by a recovery of the cerebral function at day 28 after cerebral ischemia. Taken together, these results might suggest that dopamine D(2) receptor changes in the contralateral hemisphere could have a key role in functional recovery after cerebral ischemia.
Abraham Martín; Vanessa Gómez-Vallejo; Eneko San Sebastián; Daniel Padró; Irati Markuerkiaga; Irantzu Llarena; Jordi Llop
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-14
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  33     ISSN:  1559-7016     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-01     Completed Date:  2013-03-25     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  244-52     Citation Subset:  IM    
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MeSH Terms
Brain Ischemia / metabolism*,  radiography
Dopamine Antagonists / pharmacology
Dopaminergic Neurons / metabolism*,  pathology
Fluorodeoxyglucose F18 / pharmacology
Infarction, Middle Cerebral Artery / metabolism,  radiography
Positron-Emission Tomography
Raclopride / pharmacology
Radiopharmaceuticals / pharmacology
Rats, Sprague-Dawley
Receptors, Dopamine D2
Stroke / metabolism*,  radiography
Synaptic Transmission*
Time Factors
Reg. No./Substance:
0/Dopamine Antagonists; 0/Radiopharmaceuticals; 0/Receptors, Dopamine D2; 0Z5B2CJX4D/Fluorodeoxyglucose F18; 430K3SOZ7G/Raclopride

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